构建用于单纳米精度荧光成像（FIONA）的样品室。
Constructing Sample Chambers for Fluorescence Imaging with One-Nanometer Accuracy (FIONA).
作者信息
Selvin Paul R, Lougheed Tyler, Tonks Hoffman Melinda, Park Hyokeun, Balci Hamza, Blehm Benjamin H, Toprak Erdal
机构信息
Biophysics Center and Physics Department, University of Illinois, Urbana, IL 61801, USA.
出版信息
CSH Protoc. 2007 Oct 1;2007:pdb.prot4867. doi: 10.1101/pdb.prot4867.
INTRODUCTIONFIONA, short for fluorescence imaging with one-nanometer accuracy, is a simple method for achieving localization of single (or single groups of) fluorophores with nanometer accuracy in the xy plane. This protocol provides details on constructing an inexpensive sample chamber for use in single-molecule FIONA experiments and two methods for cleaning slides and coverslips.
引言
FIONA是“具有一纳米精度的荧光成像”的缩写，是一种在xy平面上以纳米精度实现单个（或单组）荧光团定位的简单方法。本方案详细介绍了构建用于单分子FIONA实验的廉价样品室的方法，以及两种清洁载玻片和盖玻片的方法。
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