在活体系统中以单转录本分辨率对实时基因表达进行成像：构建体设计与成像系统设置。
Imaging real-time gene expression in living systems with single-transcript resolution: construct design and imaging system setup.
作者信息
Wells Amber L, Condeelis John S, Singer Robert H, Zenklusen Daniel
机构信息
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
出版信息
CSH Protoc. 2007 Nov 1;2007:pdb.top28. doi: 10.1101/pdb.top28.
Abstract
INTRODUCTIONThe most common way for a cell to respond to internal and external signals is to change its gene expression pattern. This requires the synchronization of regulatory steps along the expression pathway. Biological imaging techniques can be used to visualize and measure such processes in individual live cells in real time. This article discusses the use of a fluorescent RNA-binding protein system that allows real-time analysis of gene expression with single-transcript resolution.
摘要
引言
细胞对内部和外部信号作出反应的最常见方式是改变其基因表达模式。这需要沿表达途径的调控步骤同步进行。生物成像技术可用于实时可视化和测量单个活细胞中的此类过程。本文讨论了一种荧光RNA结合蛋白系统的应用，该系统能够以单转录本分辨率对基因表达进行实时分析。
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