Kikuta Junichi, Kawamura Shunsuke, Ishii Masaru
Laboratory of Biological Imaging, Immunology Frontier Research Center, Osaka University.
Clin Calcium. 2011 Mar;21(3):372-8.
Osteoclasts play critical roles not only in normal bone homeostasis ('remodeling') , but also in the pathogenesis of bone destructive disorders such as osteoporosis, rheumatoid arthritis, and bone metastasis. However, it has not been known how osteoclast precursor monocytes migrate into the bone surface and what controls their migratory behaviors. To reveal these systems, we have recently established a new system for visualizing intact bone tissues and bone marrow cavities in live animals by using an advanced imaging technique with intravital two-photon microscopy. By means of the system we have revealed that sphingosine-1-phosphate (S1P) , a lipid mediator, dynamically regulates migration and localization of osteoclasts and their precursors in vivo . Here we show the latest data and the detailed methodology of intravital imaging of bone tissues, and also discuss its further application.
破骨细胞不仅在正常骨稳态（“重塑”）中发挥关键作用，而且在诸如骨质疏松症、类风湿性关节炎和骨转移等骨破坏性疾病的发病机制中也起关键作用。然而，目前尚不清楚破骨细胞前体细胞单核细胞如何迁移到骨表面以及是什么控制它们的迁移行为。为了揭示这些机制，我们最近通过使用活体双光子显微镜的先进成像技术，建立了一种用于在活体动物中可视化完整骨组织和骨髓腔的新系统。借助该系统，我们发现脂质介质鞘氨醇-1-磷酸（S1P）在体内动态调节破骨细胞及其前体细胞的迁移和定位。在这里，我们展示了骨组织活体成像的最新数据和详细方法，并讨论了其进一步的应用。
