Laboratory of Biological Imaging, WPI-Immunology Frontier Research Center, Osaka University, Osaka, Japan.
Mol Cells. 2011 May;31(5):399-403. doi: 10.1007/s10059-011-1010-x. Epub 2011 Feb 25.
Sphingosine-1-phosphate (S1P), a biologically active lysophospholipid that is enriched in blood, controls the trafficking of osteoclast precursors between the circulation and bone marrow cavities via G protein-coupled receptors, S1PRs. While S1PR1 mediates chemoattraction toward S1P in bone marrow, where S1P concentration is low, S1PR2 mediates chemorepulsion in blood, where the S1P concentration is high. The regulation of precursor recruitment may represent a novel therapeutic strategy for controlling osteoclast-dependent bone remodeling. Through intravital multiphoton imaging of bone tissues, we reveal that the bidirectional function of S1P temporospatially regulates the migration of osteoclast precursors within intact bone tissues. Imaging technologies have enabled in situ visualization of the behaviors of several players in intact tissues. In addition, intravital microscopy has the potential to be more widely applied to functional analysis and intervention.
鞘氨醇-1-磷酸（S1P）是一种富含血液中的生物活性溶血磷脂，通过 G 蛋白偶联受体 S1PR 控制破骨细胞前体在血液循环和骨髓腔之间的运输。S1PR1 在 S1P 浓度较低的骨髓中介导 S1P 的趋化吸引，而 S1PR2 在 S1P 浓度较高的血液中介导趋化排斥。前体募集的调节可能代表控制破骨细胞依赖性骨重塑的一种新的治疗策略。通过骨组织的活体多光子成像，我们揭示了 S1P 的双向功能时空调节了完整骨组织中破骨细胞前体的迁移。成像技术使原位可视化完整组织中几个参与者的行为成为可能。此外，活体显微镜有可能更广泛地应用于功能分析和干预。
