Rodrigues C J, de Oliveira R M, Taniwaki N N, Bueno C, Marchiori P, Cossermelli W
Laboratory of Investigation in Therapeutic Clinic II and in Rheumatology, School of Medicine, University of São Paulo.
Rev Hosp Clin Fac Med Sao Paulo. 1990 Jul-Aug;45(4):154-7.
Epidermal nuclear deposition of immunoglobulins (in vivo ANA) was observed in 45 out of 252 skin biopsies (17.8%). It occurred in 19% of cases with systemic lupus erythematosus, in 32% of the mixed connective tissue disease, in 22% of the scleroderma, in 20% of the cutaneous vasculitis, in 18% of the polymyositis, in 33% of the Sjogren's syndrome, but it was absent in cases with rheumatoid arthritis. The in vivo ANA showed a significant association with serum antibodies to an extractable nuclear antigen (ENA), with speckled pattern of immunofluorescent antinuclear antibody (FANA) and with antibody to a fraction of ENA sensitive to ribonuclease termed ribonucleoprotein (RNP). Indirect evidence was obtained suggesting that the epidermal nuclear deposition of immunoglobulins is a true in vivo phenomenon: some patients with serum antibodies to ENA do not display in vivo ANA and contrariwise, no difference was detected between diseased and normal skin for the occurrence of in vivo ANA and also no association was observed between this phenomenon with immune deposits at dermoepidermal junction or in subepidermal vessels.
在252例皮肤活检中，45例（17.8%）观察到免疫球蛋白的表皮核沉积（体内抗核抗体）。它在19%的系统性红斑狼疮病例、32%的混合性结缔组织病病例、22%的硬皮病病例、20%的皮肤血管炎病例、18%的多发性肌炎病例、33%的干燥综合征病例中出现，但在类风湿关节炎病例中未出现。体内抗核抗体与可提取核抗原（ENA）的血清抗体、免疫荧光抗核抗体（FANA）的斑点型以及对核糖核酸酶敏感的ENA部分（称为核糖核蛋白（RNP））的抗体有显著关联。获得的间接证据表明，免疫球蛋白的表皮核沉积是一种真正的体内现象：一些有ENA血清抗体的患者未表现出体内抗核抗体，反之，在患病皮肤和正常皮肤中，体内抗核抗体的出现情况没有差异，并且该现象与真皮表皮交界处或表皮下血管中的免疫沉积物之间也未观察到关联。
