Pahor A, Krajnc I, Gorenjak M, Holc I
Department of Medicine, Rheumatology and Immunology, Maribor Teaching Hospital, Slovenia.
Wien Klin Wochenschr. 1998 May 8;110(9):338-41.
Antinuclear antibodies (ANA) are often present in connective tissue diseases. In 279 non-selected patients with connective tissue disease, inflammatory and degenerative joint disease, in some patients with chronic infectious diseases and malignancies and in the presence of some unclear pathologic conditions in patients whose serum reacted positively to ANA, we analyzed the type of immunofluorescence and the presence of extractable antinuclear antibodies (ENA). In systemic lupus erythematosus, the prevailing immunofluorescence is type H (homogenous) (60.6%), anti-Ro/SS-A appears in 24.2%, anti-Sm and anti-RNP in 12.1%. In Sjögren's syndrome, type S prevails (47.6%), anti- Ro/SS-A and anti-La/SS-B are present in 52.4%, only anti-Ro/SS-A in 28.6%. In systemic sclerosis, the prevailing immunofluorescence is type S (37.5%), in 75% a positive anti-Scl-70 antibody is present. In mixed connective tissue disease, anti-RNP appears in 85.7%. In dermatopolymyositis, the anti-Jo-1 antibody is present in 33.3%. In undifferentiated connective tissue disease, type S immunofluorescence appears in 70%. In rheumatoid arthritis the prevailing immunofluorescence is type H (homogenous) (46.4%) and type S (speckled) (41.0%), while the presence of ENA is rare (anti-Ro/SS-A in 4.6%). In spondylarthritis, type S immunofluorescence appears most often (62.5%). Patients with chronic infectious disease, malignancies, undefined conditions and degenerative joint disease present with various types of immunofluorescence; the presence of ENA is extremely rare in these patients. The results of this study underline the significance of ANA and, particularly ENA, in the diagnosis of connective tissue disease. These antibodies however, can also be identified in various infectious and malignant diseases as well as in inflammatory and degenerative joint diseases.
抗核抗体（ANA）常出现在结缔组织病中。在279例未经过挑选的患有结缔组织病、炎性和退行性关节病的患者中，部分患有慢性传染病和恶性肿瘤的患者以及一些病理情况不明但血清ANA反应呈阳性的患者，我们分析了免疫荧光类型和可提取性抗核抗体（ENA）的存在情况。在系统性红斑狼疮中，主要的免疫荧光类型是H型（均质型）（60.6%），抗Ro/SS - A出现率为24.2%，抗Sm和抗RNP为12.1%。在干燥综合征中，S型为主（47.6%），抗Ro/SS - A和抗La/SS - B的出现率为52.4%，仅抗Ro/SS - A为28.6%。在系统性硬化症中，主要的免疫荧光类型是S型（37.5%），75%的患者存在抗Scl - 70抗体阳性。在混合性结缔组织病中，抗RNP出现率为85.7%。在皮肌炎中，抗Jo - 1抗体出现率为33.3%。在未分化结缔组织病中，S型免疫荧光出现率为70%。在类风湿关节炎中，主要的免疫荧光类型是H型（均质型）（46.4%）和S型（斑点型）（41.0%），而ENA的存在较为罕见（抗Ro/SS - A为4.6%）。在脊柱关节炎中，S型免疫荧光最常出现（62.5%）。患有慢性传染病、恶性肿瘤、病情不明以及退行性关节病的患者呈现出各种类型的免疫荧光；这些患者中ENA的存在极为罕见。本研究结果强调了ANA尤其是ENA在结缔组织病诊断中的重要性。然而，这些抗体也可在各种感染性和恶性疾病以及炎性和退行性关节病中被检测到。
