Burroughs C D, Mills K T, Bern H A
Department of Zoology, University of California, Berkeley.
Reprod Toxicol. 1990;4(2):127-35. doi: 10.1016/0890-6238(90)90007-i.
The neonatal mouse model has proven to be an effective system to examine long-term reproductive tract abnormalities resulting from early exposure to estrogens. Newborn C57BL/Crgl mice received 8 x 10(-2) micrograms diethylstilbestrol (DES) or 100 micrograms coumestrol (a plant estrogen) in 0.005 mL dimethyl sulfoxide (DMSO) or DMSO alone or received no treatment for the first 5 days of life. Half of the animals were ovariectomized at 40 days of age. Vaginal lavages were examined for 15 consecutive days before termination at 13 months of age, at which time genital tracts and mammary glands were removed for histological examination. Diethylstilbestrol- and coumestrol-treated animals exhibited ovary-independent persistent vaginal cornification as well as cervico-vaginal pegs and downgrowths, uterine squamous metaplasia, and an enhancement of age-related changes in the ovary including hemorrhagic follicles. In general, neonatal exposure to the naturally occurring plant estrogen, coumestrol, has long-term effects similar to those seen following exposure to natural and synthetic estrogens.
新生小鼠模型已被证明是一种有效的系统，可用于研究早期接触雌激素所导致的长期生殖道异常情况。新生的C57BL/Crgl小鼠在出生后的前5天，每天接受0.005 mL二甲基亚砜（DMSO）中含8×10(-2)微克己烯雌酚（DES）或100微克香豆雌酚（一种植物雌激素）的处理，或仅接受DMSO处理，或不接受任何处理。一半的动物在40日龄时进行卵巢切除。在13月龄处死前，连续15天检查阴道灌洗情况，此时取出生殖道和乳腺进行组织学检查。己烯雌酚和香豆雌酚处理的动物表现出不依赖卵巢的持续性阴道角化，以及宫颈 - 阴道栓和向下生长物、子宫鳞状化生，并且卵巢中与年龄相关的变化有所增强，包括出血性卵泡。一般来说，新生期接触天然存在的植物雌激素香豆雌酚，会产生与接触天然和合成雌激素后类似的长期影响。
