Iguchi T, Ostrander P L, Mills K T, Bern H A
Department of Zoology, University of California, Berkeley 94720.
Cancer Lett. 1988 Dec 15;43(3):207-14. doi: 10.1016/0304-3835(88)90172-3.
Newborn BALB/cCrgl female mice received five daily injections of various doses of diethylstilbestrol (DES), 0.0001-10 micrograms. Mice were killed at 6 days of age or at 4 months after ovariectomy at 40-42 days. Subepithelial nodules of polygonal cells in the upper (Mullerian) vagina during early postnatal life were associated with the later occurrence of ovary-independent persistent stratification with or without cornification in mice treated neonatally with 0.1-10 micrograms DES and thus are a possible predictor of this phenomenon. The thresholds for the induction of ovary-independent epithelial pegs, downgrowths and adenosis (glandular formations) were 0.1 microgram and 0.5 microgram DES/day, respectively.
新生的BALB/cCrgl雌性小鼠每天接受五次不同剂量（0.0001 - 10微克）的己烯雌酚（DES）注射。小鼠在6日龄时处死，或在40 - 42日龄卵巢切除术后4个月处死。出生后早期，在阴道上段（苗勒管）出现的多边形细胞上皮下小结节，与新生期接受0.1 - 10微克DES处理的小鼠后期出现的不依赖卵巢的持续性分层（伴或不伴角化）有关，因此可能是这种现象的一个预测指标。诱导不依赖卵巢的上皮钉突、向下生长和腺病（腺体形成）的阈值分别为每天0.1微克和0.5微克DES。
