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外源Ca2+对高温强光胁迫下西葫芦幼苗形态、光合特性及叶绿素荧光参数的影响

[Effects of exogenous Ca2+ on morphological and photosynthetic characteristics and chlorophyll fluorescent parameters of squash seedlings under high temperature and strong light stress].

作者信息

Qin Shu-hao, Li Ling-ling, Chen Na-na

机构信息

Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2010 Nov;21(11):2830-5.

Abstract

Taking squash (Cucurbita pepo L.) variety Alan as test object, this paper studied the effects of exogenous Ca2+ on the morphological and photosynthetic characteristics and chlorophyll fluorescent parameters of squash seedlings under the cross-stress of high temperature and strong light. Under the stress, applying 5-20 mmol x L(-1) of Ca2+ increased the plant height, leaf area, chlorophyll and carotenoid contents, photosynthetic rate (Pn), stoma conductance (Gs), transpiration rate (Tr), maximal PS II efficiency (Fv/Fm), actual PS II efficiency (phi(PS II)), and photochemical queching coefficient (q(P)), and decreased the intercellular CO2 concentration (Ci) and non-photochemical fluorescence quenching coefficient (NPQ), suggesting that this application of exogenous Ca2+ could effectively mitigate the damage of high temperature and strong light stress on the squash seedlings leaf, and make it keep more rapid photosynthetic electron transfer rate and higher PS II electron transfer activity. Among the treatments of applying Ca2+, 10 mmol Ca2+ x L(-1) had the best effect. When the Ca2+ application rate exceeded 40 mmol x L(1), no mitigation effect was observed on the high temperature and strong light stress.

摘要

以西葫芦(Cucurbita pepo L.)品种“阿兰”为试验对象,研究了外源Ca2+对高温强光交叉胁迫下西葫芦幼苗形态、光合特性及叶绿素荧光参数的影响。胁迫下,施用5-20 mmol·L(-1)的Ca2+可增加株高、叶面积、叶绿素和类胡萝卜素含量、光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、最大PS II光化学效率(Fv/Fm)、实际PS II光化学效率(φ(PS II))和光化学猝灭系数(q(P)),降低胞间CO2浓度(Ci)和非光化学荧光猝灭系数(NPQ),表明施用外源Ca2+可有效减轻高温强光胁迫对西葫芦幼苗叶片的伤害,使其保持较快的光合电子传递速率和较高的PS II电子传递活性。在施用Ca2+的处理中,10 mmol·L(-1) Ca2+效果最佳。当Ca2+施用量超过40 mmol·L(-1)时,对高温强光胁迫无缓解作用。

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