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[外源脱落酸(ABA)对低温胁迫下雷公藤幼苗光合作用及叶绿素荧光参数的影响]

[Effects of exogenous abscisic acid (ABA) on the photosynthesis and chlorophyll fluorescence parameters of Tripterygium wilfordii seedlings exposed to low temperature].

作者信息

Huang Yu, Lin Zhi-Yong, Rong Jun-Dong, Chen Li-Guang, Zheng Yu-Shan

机构信息

College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2011 Dec;22(12):3150-6.

Abstract

Taking one year-old Tripterygium wilfordii cutting seedlings as test materials, this paper studied the effects of foliar spraying 0, 5, 10, 15, 20, and 25 mg x L(-1) of abscisic acid (ABA) on the leaf photosynthesis and chlorophyll fluorescence characteristics of the seedlings under low temperature stress. Spraying 20 mg x L(-1) of ABA increased the cold- resistance of the seedlings significantly, manifesting in the slowing down of the decrease amplitudes of leaf net photosynthetic rate (P(n)), transpiration rate (T(r)), stomatal conductance (g(s)), and intercellular CO2 concentration (C(i)) and the increase of photosynthetic capacity. After 6 days exposure to low temperature, the initial fluorescence (F(o)) decreased with increasing concentration of applied ABA, the maximum fluorescence (F(m)) and maximal photochemical yield (F(v)/F(m)) increased, the actual photochemical efficiency of system II (phi(PSII)) and photochemical quenching coefficient (q(P)) increased after an initial decrease, and the non-photochemical quenching coefficient (q(N)) showed a 'decreasing-increasing-decreasing' trend. The P(n), g(s), q(P), F(m), and F(v)/F(m) reached their peak values at 20 mg x L(-1) of ABA. In all treatments, with the increase of photosynthetically active radiation (PAR), the relative electron transport rate (rETR) increased first and decreased then, reached the peak when the PAR was 395 micromol x m(-2) x s(-1), and the peak value of the rETR in treatments 25 and 20 mg x L(-1) of ABA was 17.1% and 5.2% higher than that of the control, respectively. The light response curves of the psi(PSII) decreased with increasing PAR, whereas those of q(N) performed in adverse.

摘要

以一年生雷公藤扦插苗为试验材料,研究了叶面喷施0、5、10、15、20和25 mg·L⁻¹脱落酸(ABA)对低温胁迫下幼苗叶片光合作用及叶绿素荧光特性的影响。喷施20 mg·L⁻¹ABA显著提高了幼苗的抗寒性,表现为叶片净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(gs)和胞间CO₂浓度(Ci)下降幅度减缓,光合能力增强。低温处理6 d后,初始荧光(Fₒ)随ABA喷施浓度升高而降低,最大荧光(Fₘ)和最大光化学效率(Fv/Fm)升高,PSⅡ实际光化学效率(φ(PSⅡ))和光化学猝灭系数(qP)先下降后升高,非光化学猝灭系数(qN)呈“降-升-降”趋势。Pn、gs、qP、Fm和Fv/Fm在ABA浓度为20 mg·L⁻¹时达到峰值。所有处理中,随着光合有效辐射(PAR)增加,相对电子传递速率(rETR)先升后降,PAR为395 μmol·m⁻²·s⁻¹时达到峰值,25和20 mg·L⁻¹ABA处理的rETR峰值分别比对照高17.1%和5.2%。φ(PSⅡ)的光响应曲线随PAR增加而下降,qN的光响应曲线则相反。

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