Guy S P, Seabright P, Day J P, Itzhaki R F
Department of Optometry and Vision Sciences, UMIST, Manchester, United Kingdom.
J Trace Elem Electrolytes Health Dis. 1990 Sep;4(3):183-7.
We have treated human neuroblastoma cells with various complexes of aluminium, over a range of concentrations, and have measured the amount of aluminium entering the cells after one week, using atomic absorption spectroscopy. We have found that the cells incorporate much higher levels of the element after treatment with aluminium-EDTA than with aluminium-citrate or aluminium-maltol. With aluminium-EDTA, initially the uptake increases with increasing concentration in the medium but eventually reaches a plateau; the concentration within the cells is then higher than that in the medium. No obvious change in morphology of the cells occurs after treatment. The number of cells present after a week's treatment with aluminium-EDTA is lower than that of untreated cells but does not vary over a wide range of aluminium concentrations.
我们用不同浓度的各种铝配合物处理人神经母细胞瘤细胞，并在一周后使用原子吸收光谱法测量进入细胞的铝含量。我们发现，与用柠檬酸铝或麦芽醇铝处理相比，用乙二胺四乙酸铝（aluminium-EDTA）处理后，细胞摄取的该元素水平要高得多。在用乙二胺四乙酸铝处理时，最初摄取量随培养基中浓度的增加而增加，但最终会达到一个平台期；此时细胞内的浓度高于培养基中的浓度。处理后细胞形态没有明显变化。用乙二胺四乙酸铝处理一周后，细胞数量低于未处理的细胞，但在很宽的铝浓度范围内没有变化。
