Slanina P, Falkeborn Y, Frech W, Cedergren A
Food Chem Toxicol. 1984 May;22(5):391-7. doi: 10.1016/0278-6915(84)90369-7.
Male Sprague-Dawley rats were treated daily by gastric intubation (6 days/wk) with 100 mg aluminium/kg body weight in the form of aluminium hydroxide (9 wk) or aluminium citrate (4 wk), with citric acid (4 wk) or with tap-water (control, 9 wk). Young adult and aged Wistar rats were treated with 100 mg aluminium/kg body weight as aluminium hydroxide or with carboxymethylcellulose (vehicle controls). The cerebral cortex, hippocampus, cerebellum and samples of bone from each rat were analysed for aluminium, after digestion with nitric acid, using graphite furnace atomic absorption spectroscopy. The mean aluminium concentrations detected in the control Sprague-Dawley rats were 0.013-0.022 microgram/g wet weight in the various brain regions and 0.355 microgram/g in the bone. No significant increase in tissue aluminium concentrations was observed in Sprague-Dawley or Wistar rats after treatment with aluminium hydroxide. However the rats treated with aluminium citrate showed significantly increased concentrations of aluminium in all the brain regions studied (0.057-0.121 microgram A1/g) and in the bone (12.9 micrograms A1/g). Elevated aluminium concentrations in the cerebral cortex and bone were also observed in the animals fed citric acid suggesting possible absorption of the citrate chelate presumably formed with the traces of aluminium present in the diet.
将雄性斯普拉格-道利大鼠每周6天通过胃插管每日给予100毫克/千克体重的氢氧化铝形式的铝（9周）或柠檬酸铝（4周）、柠檬酸（4周）或自来水（对照组，9周）。将年轻成年和老年Wistar大鼠用100毫克/千克体重的氢氧化铝或羧甲基纤维素（赋形剂对照）进行处理。在用硝酸消化后，使用石墨炉原子吸收光谱法分析每只大鼠的大脑皮层、海马体、小脑和骨样本中的铝。在对照斯普拉格-道利大鼠中检测到的各脑区铝平均浓度为0.013 - 0.022微克/克湿重，骨中为0.355微克/克。在用氢氧化铝处理后，未观察到斯普拉格-道利大鼠或Wistar大鼠组织铝浓度有显著增加。然而，用柠檬酸铝处理的大鼠在所研究的所有脑区（0.057 - 0.121微克铝/克）和骨（12.9微克铝/克）中铝浓度显著增加。在喂食柠檬酸的动物中也观察到大脑皮层和骨中铝浓度升高，这表明可能吸收了大概与饮食中存在的痕量铝形成柠檬酸螯合物。
