Fiber-Nanotech Young Researcher Empowerment Center and Graduate School of Agriculture, Shinshu University, 8304 Minamiminowa-mura, Kamiina-gun, Nagano 399-4598 Japan.
J Agric Food Chem. 2011 Mar 23;59(6):2646-51. doi: 10.1021/jf104240d. Epub 2011 Feb 25.
Protein-energy malnutrition (PEM) is frequently associated with the occurrence of infection due to a decline in immune function. Here, an experiment was conducted with the objective of enhancing mucosal immunity by administration of Lactobacillus pentosus ONRIC b0240 (b240) in PEM model mice. Three groups of male C3H/HeN mice aged approximately 12 weeks were caged in groups of five or six and received various treatments. The mice were fed 4 (low-protein diet; PEM model), 20 (standard-protein diet), or 40% (high-protein diet) ovalbumin (OVA) with or without 0.05% b240. Five weeks later, all mice were sacrificed, and the organs were extracted for analysis of the immune response. Acute toxicity was not observed in this study. The addition of b240 showed no influence on body weight; however, body weight decreased with increasing protein level. Interestingly, intestinal total IgA was significantly increased (p<0.05) in all test diets with b240. The in vitro study showed that the number of B cells and type 2 helper T (Th2) cells were significantly increased in mouse spleen cells with b240 treatment, whereas no differences were found in the number of Th1 cells. b240 also has the ability to augment IgA and IgG production in mouse Peyer's patch cells. These results suggest that b240 enhances IgA production and helps recover the intestinal immune system in PEM model mice via augmentation of humoral immunity.
蛋白质能量营养不良（PEM）常因免疫功能下降而并发感染。本实验通过灌胃戊糖片球菌 ONRIC b0240（b240）以期增强 PEM 模型小鼠的黏膜免疫。将 3 组 12 周龄雄性 C3H/HeN 小鼠每组 5-6 只分笼饲养，分别给予不同处理：正常蛋白饮食（20%蛋白，对照）、低蛋白饮食（4%蛋白，PEM 模型）和高蛋白饮食（40%蛋白）加或不加 0.05% b240。5 周后处死小鼠，取各器官进行免疫功能分析。本实验中未观察到 b240 的急性毒性。添加 b240 对体重无影响，但随着蛋白水平的增加，体重逐渐下降。有趣的是，b240 可使所有测试饮食的肠道总 IgA 显著增加（p<0.05）。体外研究表明，b240 可使小鼠脾细胞中 B 细胞和 2 型辅助性 T 细胞（Th2）的数量显著增加，而 Th1 细胞数量无差异。b240 还可增强小鼠派伊尔氏结细胞的 IgA 和 IgG 产生。这些结果表明，b240 通过增强体液免疫增强 PEM 模型小鼠的 IgA 产生并有助于恢复其肠道免疫系统。
