Cheng Guo-Li, Wang Wei, Wang Hong-Yi, Cui Zhong-Guang
Department of Hematology, Qingdao Medical College Affiliated Hospital, Qingdao, Shandong Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2011 Feb;19(1):15-8.
This study was aimed to explore the expression of erythropoietin receptor (EPOR) on acute leukemia cells and its clinical significance. Bone marrow of 40 patients with acute leukemia (AL) and 24 patients with normal bone marrow as control group were collected. Samples came from outpatients and inpatients in our hospital. EPOR mRNA was detected by reverse transcription-PCR. The results showed that there was EPOR expression on AL cells, the expression rate was 57.5%, and the average expression level (Gray value) was 0.3549 ± 0.2800, but both were lower than that in control group (p < 0.05). There was no significant statistic difference of expression rate between acute myelogenous leukemia (AML) and acute lymphoblastic leukemia (ALL) (p > 0.05), and expression level of AML EPOR was higher than that of ALL (p < 0.05). It is concluded that there is EPOR expression on AL cells, while the expression rate and expression level are lower than those in control group (p < 0.05). There is no significant statistic difference of the expression rate between AML and ALL (p > 0.05), and the expression level of AML EPOR is higher than that of ALL (p < 0.05).
本研究旨在探讨促红细胞生成素受体（EPOR）在急性白血病细胞上的表达及其临床意义。收集40例急性白血病（AL）患者的骨髓及24例正常骨髓患者作为对照组。样本来自我院门诊及住院患者。采用逆转录聚合酶链反应（RT-PCR）检测EPOR mRNA。结果显示，AL细胞上有EPOR表达，表达率为57.5%，平均表达水平（灰度值）为0.3549±0.2800，但两者均低于对照组（p<0.05）。急性髓系白血病（AML）与急性淋巴细胞白血病（ALL）的表达率差异无统计学意义（p>0.05），AML的EPOR表达水平高于ALL（p<0.05）。结论：AL细胞上有EPOR表达，但其表达率和表达水平低于对照组（p<0.05）。AML与ALL的表达率差异无统计学意义（p>0.05），AML的EPOR表达水平高于ALL（p<0.05）。
