Tian Run-Mei, Huang Cheng-Shuang, Su Qiong, Shi Liang, Luo Xi, Chen Yan
Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi 563003，Guizhou Province, China.
Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi 563003，Guizhou Province, China,E-mail: cyz600@ 163.com.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2019 Aug;27(4):1026-1032. doi: 10.19746/j.cnki.issn.1009-2137.2019.04.006.
To investigate the expression of erythropoietin (EPO) and erythropoietin receptor (EPOR) in patients with acute leukemia (AL) and its clinical significance.
The levels of EPO and EPOR in plasma were determined by ELISA kit. mRNA expression levels of EPO and EPOR were determined by RT-RCR. The protein expression levels of EPO and EPOR were detected by Western blot.
The EPO protein levels in marrow plasma of ALL and AML group were significantly higher than those in the control group (P＜0.05), EPOR protein levels in ALL and AML group were significantly lower than those in the control group (P＜0.05). The mRNA levels of EPO and EPOR in ALL and AML groups were significantly higher than those in the control group (P＜0.05). The mRNA levels of EPO and EPOR in the high risk ALL and AML groups were significantly higher than those in the medium, low risk group and the control group (P＜0.05). The protein expression levels of EPO and EPOR in ALL and AML groups were significantly higher than that in control group (P＜0.05). The mRNA levels of EPO and EPOR in ALL and AML groups did not correlate with hemoglobin level and erythrocyte count (P＞0.05).
The expressions of EPO and EPOR is higher in ALL and AML patients. The expression levels of EPO and EPOR relate with the risk of ALL and AML. High risk patients have higher expression levels of EPO and EPOR, however, the expression levels of EPO and EPOR do not correlate with hemoglobin level and erythrocyte counting.
探讨急性白血病（AL）患者促红细胞生成素（EPO）及促红细胞生成素受体（EPOR）的表达及其临床意义。
采用ELISA试剂盒测定血浆中EPO和EPOR水平。采用RT-RCR法测定EPO和EPOR的mRNA表达水平。采用Western blot法检测EPO和EPOR的蛋白表达水平。
ALL组和AML组骨髓血浆中EPO蛋白水平显著高于对照组（P＜0.05），ALL组和AML组EPOR蛋白水平显著低于对照组（P＜0.05）。ALL组和AML组EPO和EPOR的mRNA水平显著高于对照组（P＜0.05）。高危ALL组和AML组EPO和EPOR的mRNA水平显著高于中、低危组及对照组（P＜0.05）。ALL组和AML组EPO和EPOR的蛋白表达水平显著高于对照组（P＜0.05）。ALL组和AML组EPO和EPOR的mRNA水平与血红蛋白水平及红细胞计数无相关性（P＞0.05）。
ALL和AML患者EPO和EPOR表达较高。EPO和EPOR的表达水平与ALL和AML的风险相关。高危患者EPO和EPOR表达水平较高，然而，EPO和EPOR的表达水平与血红蛋白水平及红细胞计数无相关性。
