Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, China.
Fish Shellfish Immunol. 2011 Apr-May;30(4-5):1192-6. doi: 10.1016/j.fsi.2011.02.019. Epub 2011 Mar 6.
Thioesterase superfamily member 2 (Them2) is a single hotdog fold thioesterase domain-containing protein. Its biological function is not well known. Recently, a hotdog fold thioesterase (FcThem) was cloned for the first time from the Chinese white shrimp. The full length of FcThem is 748bp. It encodes a protein with 142 amino acids with a predicted molecular mass of 14.79kDa and an isoelectric point of 8.76. No signal peptide was predicted. Multiple alignment of FcThem with other Them2 proteins suggested a conserved HGG motif. Phylogenetic analysis showed that FcThem were clustered with vertebrate Them2 protein into one group. The RT-PCR results showed that FcThem was a widely distributed gene and could be detected in the hemocytes, heart, hepatopancreas, gills, stomach, intestines, and ovaries of unchallenged shrimps. In hemocytes, its transcript was upregulated 24h post WSSV challenge. In the gills, the FcThem went up at a 6h WSSV challenge. FcThem expression in the ovaries was also affected by the WSSV and was increased after the 2h WSSV challenge, reaching the highest level at 6h. Our results show that FcThem probably has roles in the innate immunity system of shrimps and investigations will be carried out to explore this finding further.
硫酯酶超家族成员 2（Them2）是一种具有单个热狗折叠硫酯酶结构域的蛋白质。其生物学功能尚不清楚。最近，首次从中国对虾中克隆出一种热狗折叠硫酯酶（FcThem）。FcThem 的全长为 748bp，编码一个由 142 个氨基酸组成的蛋白质，预测分子量为 14.79kDa，等电点为 8.76。未预测到信号肽。FcThem 与其他 Them2 蛋白的多重比对表明存在保守的 HGG 基序。系统发育分析表明，FcThem 与脊椎动物 Them2 蛋白聚类为一组。RT-PCR 结果表明，FcThem 是一种广泛分布的基因，可在未受挑战的虾的血细胞、心脏、肝胰腺、鳃、胃、肠和卵巢中检测到。在血细胞中，其转录本在 WSSV 挑战后 24h 上调。在鳃中，FcThem 在 6h WSSV 挑战时上升。WSSV 也影响卵巢中的 FcThem 表达，并在 2h WSSV 挑战后增加，在 6h 时达到最高水平。我们的结果表明，FcThem 可能在虾的先天免疫系统中发挥作用，我们将进一步开展研究以探索这一发现。
