Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, China.
Dev Comp Immunol. 2011 Jun;35(6):629-34. doi: 10.1016/j.dci.2011.01.004. Epub 2011 Jan 14.
Receptor for activated C kinase 1 (RACK1), which has seven tandem WD40 domains, is a scaffolding protein. RACK1 plays different roles by binding to different partner proteins. It is involved in hormone signaling and development, and now some evidence indicates it may have a role in innate immunity. In this paper, RACK1 cDNA from Chinese white shrimp (FcRACK1) was identified. The full length of the FcRACK1 gene is 1037 bp, including a 30 bp 5'UTR, a 957 bp ORF encoding a 318 amino acid protein, and a 50 bp 3'UTR with the polyadenylation sequence AATAAA and a poly (A) tail. The FcRACK1 protein is characterized by seven WD40 repeat domains; the ending two amino acids of each WD40 domain are WK, WD, WN, WS, WD, WD, and WQ, respectively. The length of each domain is between 30 and 44 amino acids. Multiple alignments of RACK1s showed that RACK1s are highly conserved. RT-PCR showed that FcRACK1 could be detected in hemocytes, the heart, hepatopancreas, gills, stomach, intestine, and ovary. FcRACK1 in hemocytes was down-regulated after a 2h WSSV challenge, and FcRACK1 in gills was up-regulated after a 2 h Vibrio challenge. FcRACK1 in ovary went down after a 12 h Vibrio challenge and then up-regulated at 24 h. FcRACK1 in ovary was first down-regulated at 2 h after a WSSV challenge and then up-regulated to the highest level at 6 h. It finally went down from 12 to 24 h. In hepatopancreas, FcRACK1 was also up-regulated by microbe challenge. Our results indicated its probable role in shrimp innate immunity.
激活蛋白激酶 C 受体 1（RACK1）具有七个串联 WD40 结构域，是一种支架蛋白。RACK1 通过与不同的伴侣蛋白结合而发挥不同的作用。它参与激素信号转导和发育，现在有一些证据表明它可能在先天免疫中发挥作用。在本文中，鉴定了中国对虾（FcRACK1）的 RACK1 cDNA。FcRACK1 基因全长 1037bp，包括 30bp5'UTR、957bpORF 编码 318 个氨基酸的蛋白和 50bp3'UTR，其中包含 polyadenylation 序列 AATAAA 和 poly（A）尾。FcRACK1 蛋白的特征是七个 WD40 重复结构域；每个 WD40 结构域的最后两个氨基酸分别为 WK、WD、WN、WS、WD、WD 和 WQ。每个结构域的长度在 30 到 44 个氨基酸之间。RACK1 之间的多重比对表明 RACK1 高度保守。RT-PCR 显示 FcRACK1 可在血细胞、心脏、肝胰腺、鳃、胃、肠和卵巢中检测到。血细胞中的 FcRACK1 在 WSSV 挑战 2 小时后下调，鳃中的 FcRACK1 在 Vibrio 挑战 2 小时后上调。Vibrio 挑战 12 小时后卵巢中的 FcRACK1 下调，24 小时后上调。VSSV 挑战 2 小时后卵巢中的 FcRACK1 首先下调，然后在 6 小时上调至最高水平，最后在 12 至 24 小时下调。肝胰腺中的 FcRACK1 也受到微生物挑战的上调。我们的结果表明它可能在虾的先天免疫中发挥作用。
