Hangzhou Key Laboratory of Animal Adaptation and Evolution, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Comp Biochem Physiol B Biochem Mol Biol. 2011 Jun;159(2):92-102. doi: 10.1016/j.cbpb.2011.02.005. Epub 2011 Mar 23.
To gain insight into the comparative function in stress response of HSPs in insects, three HSP cDNAs were cloned from the fat body of the beet armyworm Spodoptera exigua (Lepidoptera, Noctuidae). SexHSP70, SexHSP74 and SexHSP83 cDNAs encoding the protein of 667, 685 and 717 amino acids, with the pI of 5.52, 5.75 and 5.02, respectively. Northern blotting revealed that all three SexHSP mRNAs are expressed in the fat body, mid-gut, spermary and tracheae. SexHSP70, SexHSP74and SexHSP83 mRNAs were expressed in the fat body and whole body at different levels during different developmental stages. The three SexHSP transcripts were highly expressed in the fat body on the first day of fifth instar larvae, on the fourth and seventh days of the pupa stage, and in the whole body on the initial stages of larvae. Under heat and cold shock conditions, SexHSP70 and SexHSP83 mainly functioned during heat shock and cooling and SexHSP83 also had a function in the recovery stage. SexHSP74 had important functions in short-term heat shock and recovery, as well as long-term cooling. The results revealed that long-term shocking can affect SexHSP74 and SexHSP83 expression and long-term cooling can influence SexHSP83 expression during the recovery stage.
为了深入了解昆虫热休克蛋白在应激反应中的比较功能，我们从甜菜夜蛾（鳞翅目夜蛾科）的脂肪体中克隆了三个 HSP cDNA。SexHSP70、SexHSP74 和 SexHSP83 cDNA 分别编码 667、685 和 717 个氨基酸的蛋白质，等电点分别为 5.52、5.75 和 5.02。Northern blot 分析表明，这三种 SexHSP mRNA 均在脂肪体、中肠、精巢和气管中表达。SexHSP70、SexHSP74 和 SexHSP83 mRNA 在不同发育阶段的脂肪体和整个体内均有不同水平的表达。在第五龄幼虫的第一天、蛹期的第四天和第七天以及幼虫初期，这三种 SexHSP 转录本在脂肪体中高度表达。在热激和冷激条件下，SexHSP70 和 SexHSP83 主要在热激和冷却时发挥作用，SexHSP83 在恢复阶段也有作用。SexHSP74 在短期热激和恢复以及长期冷却中具有重要作用。结果表明，长期冲击会影响 SexHSP74 和 SexHSP83 的表达，而长期冷却会影响恢复阶段的 SexHSP83 表达。
