Hanisch F G, Peter-Katalinic J, Egge H, Dabrowski U, Uhlenbruck G
Institut für Immunbiologie, Universitätskliniken Köln, Germany.
Glycoconj J. 1990;7(6):525-43. doi: 10.1007/BF01189075.
O-Linked glycans were isolated from human skim milk mucins or mucin-derived high-molecular weight glycopeptides and fractionated by anion exchange chromatography into neutral and acidic alditols. Major oligosaccharides contained in the acidic fraction were purified by high performance liquid chromatography and structurally characterized by a combination of fast atom bombardment mass spectrometry, methylation analysis and 500 MHz 1H-nuclear magnetic resonance spectroscopy. The structural aspects exhibited by these major species in the acidic fraction resemble those established previously for the neutral oligosaccharides from human skim milk mucins: 1) the size of the alditols varies from tri- to decasaccharides, 2) the core structure is of the ubiquitous type 2, 3) the backbone sequences are of the poly-N-acetyllactosamine type with a particular preponderance of linearly extended GlcNAc beta(1-3)Gal (major) or GlcNAc beta(1-6)Gal units (minor).
O-连接聚糖从人脱脂乳粘蛋白或粘蛋白衍生的高分子量糖肽中分离出来，并通过阴离子交换色谱法分离成中性和酸性糖醇。酸性部分中含有的主要寡糖通过高效液相色谱法纯化，并通过快原子轰击质谱、甲基化分析和500 MHz 1H-核磁共振光谱相结合的方法进行结构表征。这些酸性部分中的主要种类所呈现的结构特征类似于先前从人脱脂乳粘蛋白中确定的中性寡糖的结构特征：1）糖醇的大小从三糖到十糖不等，2）核心结构是普遍存在的2型，3）主链序列是聚N-乙酰乳糖胺型，其中线性延伸的GlcNAcβ(1-3)Gal（主要）或GlcNAcβ(1-6)Gal单元（次要）占特别优势。
