Stem Cell and Gene Therapy Research and Application Center, Kocaeli University, Kocaeli, Turkey.
Cell Death Dis. 2010 Apr 22;1(4):e36. doi: 10.1038/cddis.2010.14.
The maintenance of viable and functional islets is critical in successful pancreatic islet transplantation from cadaveric sources. During the isolation procedure, islets are exposed to a number of insults including ischemia, oxidative stress and cytokine injury that cause a reduction in the recovered viable islet mass. A novel approach was designed in which streptozotocin (STZ)-damaged rat pancreatic islets (rPIs) were indirectly cocultured with rat bone marrow-derived mesenchymal stem cells (rBM-MSCs) to maintain survival of the cultured rPIs. The results indicated that islets cocultured with rBM-MSCs secreted an increased level of insulin after 14 days, whereas non-cocultured islets gradually deteriorated and cell death occurred. The cocultivation of rBM-MSCs with islets and STZ-damaged islets showed the expression of IL6 and transforming growth factor-β1 in the culture medium, besides the expression of the antiapoptotic genes (Mapkapk2, Tnip1 and Bcl3), implying the cytoprotective, anti-inflammatory and antiapoptotic effects of rBM-SCs through paracrine actions.
从尸体来源成功进行胰岛移植的关键是维持有活力和功能的胰岛。在分离过程中，胰岛会受到多种损伤，包括缺血、氧化应激和细胞因子损伤，这会导致回收的有活力胰岛质量减少。本研究设计了一种新方法，即间接共培养链脲佐菌素（STZ）损伤的大鼠胰岛（rPIs）与大鼠骨髓间充质干细胞（rBM-MSCs），以维持培养的 rPIs 的存活。结果表明，与 rBM-MSCs 共培养的胰岛在 14 天后分泌的胰岛素水平增加，而非共培养的胰岛逐渐恶化并发生细胞死亡。rBM-MSCs 与胰岛和 STZ 损伤的胰岛共培养显示培养基中表达了白细胞介素 6 和转化生长因子-β1，以及抗凋亡基因（Mapkapk2、Tnip1 和 Bcl3）的表达，表明 rBM-SCs 通过旁分泌作用具有细胞保护、抗炎和抗凋亡作用。
