Arbo A, Hoefsloot M, Ramírez A, Ignacio Santos J
Depto. de Enfermedades Infecciosas y Parasitarias, Hospital Infantil de México Federico Gómez, D.F.
Arch Invest Med (Mex). 1990;21 Suppl 1:57-61.
Since toxic oxygen metabolites generated during the respiratory burst of polymorphonuclear neutrophils (PMNs) constitute one of the most potent microbicidal mechanisms of PMNs, we evaluated the in vitro capacity of E histolytica to trigger the respiratory burst of human PMNs using a luminol-enhanced chemiluminescence assay a highly virulent strain of E. histolytica (HM1-IMSS) and a less virulent one (HK9-NIH) were incubated with PMNs at 4:1, 2:1 and 1:1 PMN:amoebae ratios. We have observed that the HM1-IMSS strain of E. histolytica do not induce PMN chemiluminescence as compared with zymosan (4.57.1.1 vs 106.5.11.3 mV/2 x 10(5) PMN, p less than 0.001). More importantly, the neutrophil oxidative response decreased progressively when the concentration of amoebae in the reaction mixture increased. When PMNs were challenged with zymosan in the presence of amoebae, the response was abrogated. These results suggest that E. histolytica inhibits the respiratory burst of PMNs. This represents a unique survival tactic and may contribute to the pathogenesis of amebiasis.
由于多形核中性粒细胞（PMN）呼吸爆发过程中产生的有毒氧代谢产物是PMN最有效的杀菌机制之一，我们使用鲁米诺增强化学发光测定法评估了溶组织内阿米巴在体外触发人PMN呼吸爆发的能力。将高毒力的溶组织内阿米巴菌株（HM1-IMSS）和低毒力的菌株（HK9-NIH）与PMN以4:1、2:1和1:1的PMN:变形虫比例孵育。我们观察到，与酵母聚糖相比，溶组织内阿米巴的HM1-IMSS菌株不会诱导PMN化学发光（4.57±1.1对106.5±11.3 mV/2×10⁵ PMN，p<0.001）。更重要的是，当反应混合物中变形虫的浓度增加时，中性粒细胞的氧化反应逐渐降低。当在存在变形虫的情况下用酵母聚糖攻击PMN时，反应被消除。这些结果表明，溶组织内阿米巴抑制PMN的呼吸爆发。这代表了一种独特的生存策略，可能有助于阿米巴病的发病机制。
