Guerrant R L, Brush J, Ravdin J I, Sullivan J A, Mandell G L
J Infect Dis. 1981 Jan;143(1):83-93. doi: 10.1093/infdis/143.1.83.
The interaction of human polymorphonuclear neutrophils (PMNs) with two strains of Entamoeba histolytica that exhibit different degrees of "virulence" was examined in animal and tissue culture systems. PMNs actively migrated toward both strains of amoebae. Less virulent amoebae (strain 303) were surrounded by PMNs (greater than or equal to 300/amoeba), fragmented and ingested by PMNs by means that appeared to be independent of oxygen. In contrast, contact with virulent amoebae (HM1) caused loss of PMN motility, PMN degranulation, death, and occasional phagocytosis by the amoebae, which survived concentrations of greater than 1,000 PMNs/amoeba. PMNs that did not directly contact virulent amoebae remained alive and active. At least two separate components of cytopathogenicity by virulent amoebae were observed: first, extracellular, contact-dependent, serum-independent cytolysis that was dependent on intact microfilament function; and second, amoeba phagocytosis of human PMNs. This contact-dependent ability of virulent amoebae to kill and phagocytize host neutrophils may be important in their pathogenic capacity.
在动物和组织培养系统中，研究了人类多形核中性粒细胞（PMN）与两株表现出不同程度“毒力”的溶组织内阿米巴的相互作用。PMN会主动向这两株阿米巴移动。毒力较弱的阿米巴（303株）被PMN包围（每只阿米巴周围有300个及以上PMN），被PMN碎片化并吞噬，其方式似乎与氧气无关。相比之下，与毒力强的阿米巴（HM1）接触会导致PMN失去运动能力、PMN脱颗粒、死亡，且阿米巴偶尔会进行吞噬作用，在每只阿米巴周围有超过1000个PMN的浓度下仍能存活。未直接接触毒力强的阿米巴的PMN仍保持存活和活性。观察到毒力强的阿米巴至少有两个独立的细胞致病性成分：第一，细胞外、接触依赖性、血清非依赖性细胞溶解，这依赖于完整的微丝功能；第二，阿米巴对人类PMN的吞噬作用。毒力强的阿米巴这种接触依赖性杀死和吞噬宿主中性粒细胞的能力可能在其致病能力中起重要作用。
