University of São Paulo.
J Appl Oral Sci. 2004 Jun;12(2):99-103. doi: 10.1590/s1678-77572004000200003.
The purpose of this study was to evaluate bacterial survival rate on toothbrushes after brushing and the efficacy of their decontamination by spraying antimicrobial solutions. Thirty subjects were instructed to spray the solutions on toothbrush bristles after brushing. Each volunteer tested three sprays, one solution per week; the sprays were labeled spray 1 (cetylpyridinium chloride - CPC - and basic formulation), 2 (basic formulation only) and 3 (control - sterile tap water). At the end of each week, the brushes were collected and sonicated in Letheen Broth®; the suspensions were ten-fold diluted and the dilutions were plated on various culture media. Anaerobic bacteria, evaluated by colony count of black pigment producing organisms on Ask medium, were recovered from 83.3% of the samples, Streptococci from 80% and aerobic Gram-negative bacilli from 46.7% of them in the control tests. There was a significant decrease in toothbrush contamination with antimicrobial sprays 1 and 2, the first showing the greatest decrease on bacterial counts.
本研究旨在评估刷牙后牙刷上细菌的存活率，以及喷洒抗菌溶液对其消毒效果。30 名受试者被指示在刷牙后将溶液喷洒在牙刷刷毛上。每位志愿者每周测试三种喷雾，一种溶液一种喷雾；喷雾分别标记为喷雾 1（十六烷基吡啶氯-CPC-和基础配方）、2（仅基础配方）和 3（对照-无菌自来水）。每周结束时，收集牙刷并在 Letheen Broth®中进行超声处理；将悬浮液进行十倍稀释，将稀释液接种在各种培养基上。在对照试验中，从 83.3%的样本中回收了产黑色色素的厌氧细菌的菌落计数，从 80%的样本中回收了链球菌，从 46.7%的样本中回收了需氧革兰氏阴性杆菌。使用抗菌喷雾 1 和 2 后，牙刷的污染明显减少，第一种喷雾对细菌计数的减少最大。
