Meier S, Collier C, Scaletta M G, Stephens J, Kimbrough R, Kettering J D
Dental Hygiene Department, School of Dentistry, Loma Linda University, California, USA.
J Dent Hyg. 1996 Jul-Aug;70(4):161-5.
A product designed as a toothbrush disinfectant containing cetylpyridinium chloride (CPC), a quaternary ammonium compound, recently was introduced. The purpose of this study was to provide additional evidence that CPC provides a practical solution for destroying residual microorganisms on air-dried toothbrushes and toothbrushes stored in a travel container.
Sterile synthetic toothbrushes were inoculated with optical density standardized laboratory cultures of Staphylococcus epidermidis or Candida albicans. Half were then disinfected with CPC and half were used as untreated controls. The toothbrushes were vortexed in sterile saline solution, diluted in a ten-fold series, and plated on 5% blood agar or Sabouraud dextrose agar. The plates were incubated at 37 degrees C in a normal atmosphere for 48 hours, and colonies were counted.
CPC produced significant decreases in residual microorganisms. Using the CPC spray treatment on air-dried toothbrushes, Staphylococcus epidermidis essentially was reduced 100-fold, while Candida albicans had a 94% reduction of growth. Bacterial counts were higher in the samples stored in closed containers as compared to the air-dried samples.
CPC appeared to be an effective toothbrush disinfectant for the organisms evaluated. It is practical and economical. CPC could easily fit into the recommendations of a practice committed to infection control.
最近推出了一种设计为含季铵化合物十六烷基氯化吡啶（CPC）的牙刷消毒剂产品。本研究的目的是提供更多证据，证明CPC为消灭风干牙刷和储存在旅行容器中的牙刷上的残留微生物提供了切实可行的解决方案。
用表皮葡萄球菌或白色念珠菌的光密度标准化实验室培养物接种无菌合成牙刷。然后将一半牙刷用CPC消毒，另一半用作未处理的对照。将牙刷在无菌盐溶液中涡旋，进行十倍系列稀释，然后接种于5%血琼脂或沙氏葡萄糖琼脂平板上。平板在37℃的正常大气中孵育48小时，然后计数菌落。
CPC使残留微生物显著减少。对风干牙刷使用CPC喷雾处理后，表皮葡萄球菌数量基本减少了100倍，而白色念珠菌的生长减少了94%。与风干样本相比，储存在密闭容器中的样本中的细菌计数更高。
对于所评估的微生物，CPC似乎是一种有效的牙刷消毒剂。它既实用又经济。CPC可以很容易地纳入致力于感染控制的实践建议中。
