Center for Geo-Environmental Science, Graduate School of Engineering and Resource Science, Akita University, 1-1 Tegata Gakuen-machi, Akita 010-8502, Japan.
J Mater Sci Mater Med. 2011 Apr;22(4):977-87. doi: 10.1007/s10856-011-4267-x. Epub 2011 Mar 2.
The materials (C-ODTi) with different topographical surfaces that possess interstitial oxygen atoms into the host titanium lattice and an upper nanometric surface layer of anatase-TiO(2) covered by a carbon thin layer were fabricated in this study. The carbon thin layer on the surface of C-ODTi was composed of amorphous carbon and nano-graphite crystals. In vitro tests, using human bone marrow-derived mesenchymal cells (hBMCs), were performed to check cytotoxicity, examining in particular cell morphology, cell proliferation, cell differentiation, and mineralization capability. After 10 days of culture a higher degree of cell viability was observed on the surface of C-ODTi with an abraded surface. We also observed that hBMCs cultured in direct contact with C-ODTi maintained their capability to express alkaline phosphatase activity (ALP) and formed mineralized nodules similar to the control cultures. Our results demonstrate that the carbon layer coating on the surface of C-ODTi possess better biological response than commercially pure titanium (cp Ti), which was evidenced by the higher proliferation rates of osteoblasts, higher osteo-differentiation and a higher mineralization capability.
本研究制备了具有不同形貌表面的材料（C-ODTi），其特征在于主体钛晶格中存在间隙氧原子和覆盖有碳薄层的锐钛矿 TiO(2) 的上纳米级表面层。C-ODTi 表面的碳薄层由无定形碳和纳米石墨晶体组成。使用人骨髓间充质细胞（hBMCs）进行体外测试，以检查细胞毒性，特别检查细胞形态、细胞增殖、细胞分化和矿化能力。培养 10 天后，在表面经过研磨的 C-ODTi 上观察到更高的细胞活力。我们还观察到，与 C-ODTi 直接接触培养的 hBMCs 保持表达碱性磷酸酶活性（ALP）的能力，并形成类似于对照培养物的矿化结节。我们的结果表明，C-ODTi 表面的碳层涂层具有比商业纯钛（cp Ti）更好的生物学反应，这表现在成骨细胞的增殖率更高、骨分化程度更高和矿化能力更强。
