Dean Jonathan, Ta Thi Thu Hong, Dunford Linda, Carr Michael J, Nguyen Linh Thuy, Coughlan Suzie, Connell Jeff, Nguyen Hien Tran, Hall William W, Nguyen Thi Lan Anh
Ireland Vietnam Blood-Borne Virus Initiative, Dublin, Ireland.
AIDS Res Hum Retroviruses. 2011 Jul;27(7):797-801. doi: 10.1089/aid.2011.0013. Epub 2011 Mar 31.
The prevalence of HIV-1 drug resistance mutations (DRMs) was determined for a cross-section of individuals (n=8654) in five centers across Vietnam (Hanoi, Hai Phong, Da Nang, Khanh Hoa, and Can Tho) between 2008 and 2009. Following serological screening for HIV infection, HIV-1 viral load was determined, using an in-house real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay. Samples with quantifiable viral loads [all either commercial sex workers (CSW) or intravenous drug users (IDU)] underwent DRM analysis. Sequences were obtained for 92 treatment-naive individuals, the majority of whom were infected with HIV-1 CRF01_AE (99%), with one instance of subtype A1 also detected. DRMs were detected in seven treatment-naive individuals (7.6%). The most common DRMs observed were M184V, V75A/M, M41L, and K65R (NRTI) and K103N, G190A, and Y181C (NNRTI). Overall, the data from this first multicenter survey of DRMs in Vietnam indicate that the problem of transmitted drug resistance is of major concern in the highest-risk groups of IDU and CSW.
2008年至2009年期间，对越南五个中心（河内、海防、岘港、庆和、芹苴）的一组个体（n = 8654）进行了HIV-1耐药性突变（DRM）流行率的测定。在对HIV感染进行血清学筛查后，使用内部实时逆转录聚合酶链反应（RT-PCR）检测法测定HIV-1病毒载量。对病毒载量可量化的样本（均为商业性工作者或静脉吸毒者）进行DRM分析。对92名未经治疗的个体进行了测序，其中大多数感染了HIV-1 CRF01_AE（99%），还检测到1例A1亚型感染。在7名未经治疗的个体中检测到DRM（7.6%）。观察到的最常见DRM为M184V、V75A/M、M41L和K65R（核苷类逆转录酶抑制剂）以及K103N、G190A和Y181C（非核苷类逆转录酶抑制剂）。总体而言，越南首次多中心DRM调查的数据表明，传播性耐药问题在静脉吸毒者和商业性工作者等高危人群中是一个主要问题。
