Do Hung Thai, Nguyen Dong Thanh, Nguyen Lan Anh Thi, Do Duong Huy, Le Huy Xuan, Trinh Xuan Mai Thi, Ton Hong Vy Nu, Sawada Ikumi, Kitamura Noriko, Le Minh Nhat, Yoshihara Keisuke, Phan Thu Huong Thi, Bui Chien Trong, Ariyoshi Koya, Yoshida Lay Myint
Pasteur Institute in Nha Trang.
Institute of Tropical Medicine, Nagasaki University.
Jpn J Infect Dis. 2017 Nov 22;70(6):621-627. doi: 10.7883/yoken.JJID.2016.512. Epub 2017 Sep 11.
We studied the prevalence of HIV drug resistance among high-risk groups such as injecting drug users (IDUs), female sex workers (FSWs), and men having sex with men (MSM) in central Vietnam. We used HIV-positive blood samples from 2012-2013 sentinel surveillance surveys. Study subjects were screened for HIV infection by standardized screening assays, and the HIV-positive samples were further tested for HIV viral load and drug-resistance mutations (DRMs) by in-house assays. DRMs were identified using the Stanford University online sequence analysis tool. Their risk behaviors were also investigated. During the study period, 6,016 (high-risk) subjects were screened, and 97 tested positive for HIV infection (IDUs: n = 63, 3.0%; FSWs: n = 24, 0.9%; and MSM: n = 10, 1.0%). Ninety-two of the 97 samples (45 from 2012 and 47 from 2013) were available for further testing. HIV RNA was detected in 56 (60.9%) of the 92 samples, and drug resistance genotyping was successfully performed on 40 (71.4%) samples. All these isolates were subtype CRF01_AE, except for 1 (2.5%) IDU whose HIV belongs to subtype B. Thirteen individuals (32.5%) were carrying HIV with at least 1 DRM: 9 IDUs, 1 FSW, and 3 MSM. Thus, HIV seroprevalence among high-risk individuals in central Vietnam is low, but a high proportion of drug resistant HIV-1 isolates is observed in the high-risk group.
我们研究了越南中部注射吸毒者（IDU）、女性性工作者（FSW）和男男性行为者（MSM）等高风险人群中艾滋病毒耐药性的流行情况。我们使用了2012 - 2013年哨点监测调查中的艾滋病毒阳性血液样本。通过标准化筛查检测对研究对象进行艾滋病毒感染筛查，对艾滋病毒阳性样本通过内部检测进一步检测艾滋病毒病毒载量和耐药突变（DRM）。使用斯坦福大学在线序列分析工具鉴定DRM。还对他们的风险行为进行了调查。在研究期间，共筛查了6016名（高风险）对象，其中97人艾滋病毒检测呈阳性（注射吸毒者：n = 63，3.0%；女性性工作者：n = 24，0.9%；男男性行为者：n = 10，1.0%）。97个样本中的92个（2012年45个，2013年47个）可用于进一步检测。92个样本中有56个（60.9%）检测到艾滋病毒RNA，40个（71.4%）样本成功进行了耐药基因分型。除1名（2.5%）注射吸毒者的艾滋病毒属于B亚型外，所有这些分离株均为CRF01_AE亚型。13人（32.5%）携带的艾滋病毒至少有1种DRM：9名注射吸毒者、1名女性性工作者和3名男男性行为者。因此，越南中部高风险人群中的艾滋病毒血清流行率较低，但在高风险组中观察到高比例的耐药艾滋病毒-1分离株。
