Bhatti G K, Bhatti J S, Kiran R, Sandhir R
Panjab University, Department of Biochemistry, Basic Medical Sciences Building, Chandigarh, India.
Cell Mol Biol (Noisy-le-grand). 2011 Feb 12;57(1):70-9.
Erythrocyte membranes are an excellent model system to study interaction of pro-oxidants with membranes. The aim of the present study is to examine the effect of vitamin E on ethion-induced biochemical and morphological alterations in erythrocytes. Ethion was administered to the rats orally at a daily dose of 2.7 mg/kg body weight for a period of 7, 14, 21 and 28 days. The results from the present study show that administration of ethion resulted in oxidative damage to erythrocyte membranes as evident by increased lipid peroxidation and decreased phospholipid content. This was accompanied by decrease in membrane cholesterol levels. In addition, ethion exposure inhibited the activities of membrane bound enzymes; Na+ K+ ATPase and Mg2+ATPase. Scanning electron micrographs of erythrocytes from animals exposed to ethion revealed morphological changes. Supplementation of vitamin E (50 mg/kg body weight) to ethion exposed animals ameliorated the ethion-induced oxidative stress, restored membrane lipid composition and activity of membrane bound enzymes along with erythrocyte shape. The results clearly demonstrate that ethion-induced damage involves increase in oxidative stress that results in alterations in erythrocyte membrane structure and function. Furthermore, supplementation with vitamin E reversed ethion induced alterations suggesting its beneficial role in individuals exposed to ethion.
红细胞膜是研究促氧化剂与膜相互作用的理想模型系统。本研究的目的是检测维生素E对乙硫磷诱导的红细胞生化和形态改变的影响。以每日2.7毫克/千克体重的剂量给大鼠口服乙硫磷，持续7、14、21和28天。本研究结果表明，乙硫磷的施用导致红细胞膜发生氧化损伤，脂质过氧化增加和磷脂含量降低证明了这一点。这伴随着膜胆固醇水平的降低。此外，乙硫磷暴露抑制了膜结合酶Na+K+ATP酶和Mg2+ATP酶的活性。对暴露于乙硫磷的动物的红细胞进行扫描电子显微镜检查发现了形态变化。给暴露于乙硫磷的动物补充维生素E（50毫克/千克体重）可改善乙硫磷诱导的氧化应激，恢复膜脂质组成和膜结合酶的活性以及红细胞形状。结果清楚地表明，乙硫磷诱导的损伤涉及氧化应激增加，从而导致红细胞膜结构和功能改变。此外，补充维生素E可逆转乙硫磷诱导的改变，表明其对暴露于乙硫磷的个体具有有益作用。
