College of Marine Science, University of South Florida, St Petersburg, FL 33701, USA.
School of Biological Sciences, University of Canterbury, Ilam, Christchurch 8140, New Zealand.
J Gen Virol. 2011 Jun;92(Pt 6):1302-1308. doi: 10.1099/vir.0.030338-0. Epub 2011 Mar 2.
Dragonfly cyclovirus (DfCyV), a new species of ssDNA virus discovered using viral metagenomics in dragonflies (family Libellulidae) from the Kingdom of Tonga. Metagenomic sequences of DfCyV were similar to viruses of the recently proposed genus Cyclovirus within the family Circoviridae. Specific PCRs resulted in the recovery of 21 DfCyV genomes from three dragonfly species (Pantala flavescens, Tholymis tillarga and Diplacodes bipunctata). The 1741 nt DfCyV genomes share >95 % nucleotide identity and are classified into 11 subtypes representing a single strain. The DfCyV genomes share 48-63 % genome-wide nucleotide identity with cycloviruses identified in human faecal samples. Recombination analysis revealed three recombinant DfCyV genomes, suggesting that recombination plays an important role in cyclovirus evolution. To our knowledge, this is the first report of a circular ssDNA virus identified in insects, and the data may help elucidate evolutionary links among novel Circoviridae recently identified in animals and environmental samples.
蜻蜓环病毒（DfCyV），一种使用在来自汤加王国的蜻蜓（蜻蜓科）中的病毒宏基因组学发现的新型 ssDNA 病毒。DfCyV 的宏基因组序列与最近提出的环病毒属中的 Circoviridae 家族中的病毒相似。特定的 PCR 导致从三种蜻蜓物种（Pantala flavescens、Tholymis tillarga 和 Diplacodes bipunctata）中回收了 21 个 DfCyV 基因组。1741nt 的 DfCyV 基因组具有 >95%的核苷酸同一性，并被分为 11 个亚型，代表一个单一的株系。DfCyV 基因组与在人类粪便样本中鉴定出的环病毒具有 48-63%的全基因组核苷酸同一性。重组分析显示了三个重组的 DfCyV 基因组，表明重组在环病毒进化中起着重要作用。据我们所知，这是首次在昆虫中发现的圆形 ssDNA 病毒的报道，这些数据可能有助于阐明最近在动物和环境样本中鉴定出的新型 Circoviridae 之间的进化联系。
