病毒宏基因组学揭示了林麝粪便中多样化的 CRESS-DNA 病毒基因组。
Viral metagenomics revealed diverse CRESS-DNA virus genomes in faeces of forest musk deer.
机构信息
Department of Microbiology, School of Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China.
Agricultural Engineering Research Institute, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
出版信息
Virol J. 2020 Apr 25;17(1):61. doi: 10.1186/s12985-020-01332-y.
BACKGROUND
Musk deer can produce musk which has high medicinal value and is closely related to human health. Viruses in forest musk deer both threaten the health of forest musk deer and human beings.
METHODS
Using viral metagenomics we investigated the virome in 85 faeces samples collected from forest musk deer.
RESULTS
In this article, eight novel CRESS-DNA viruses were characterized, whole genomes were 2148 nt-3852 nt in length. Phylogenetic analysis indicated that some viral genomes were part of four different groups of CRESS-DNA virus belonging in the unclassified CRESS-DNA virus, Smacoviridae, pCPa-like virus and pPAPh2-like virus. UJSL001 (MN621482), UJSL003 (MN621469) and UJSL017 (MN621476) fall into the branch of unclassified CRESS-DNA virus (CRESSV1-2), UJSL002 (MN621468), UJSL004 (MN621481) and UJSL007 (MN621470) belong to the cluster of Smacoviridae, UJSL005 (MN604398) showing close relationship with pCPa-like (pCRESS4-8) clusters and UJSL006 (MN621480) clustered into the branch of pPAPh2-like (pCRESS9) virus, respectively.
CONCLUSION
The virome in faeces samples of forest musk deer from Chengdu, Sichuan province, China was revealed, which further characterized the diversity of viruses in forest musk deer intestinal tract.
背景
麝鹿能够产生麝香，麝香具有很高的药用价值，与人类健康密切相关。森林麝鹿体内的病毒既威胁着森林麝鹿的健康，也威胁着人类的健康。
方法
我们利用病毒宏基因组学技术，对来自中国四川成都的 85 份麝鹿粪便样本中的病毒组进行了研究。
结果
本文描述了 8 种新型的 CRESS-DNA 病毒，其基因组全长为 2148nt-3852nt。系统进化分析表明，部分病毒基因组属于 4 个不同的 CRESS-DNA 病毒群，分别为未分类的 CRESS-DNA 病毒、Smacoviridae 病毒、pCPa-like 病毒和 pPAPh2-like 病毒。UJSL001（MN621482）、UJSL003（MN621469）和 UJSL017（MN621476）属于未分类的 CRESS-DNA 病毒（CRESSV1-2）分支，UJSL002（MN621468）、UJSL004（MN621481）和 UJSL007（MN621470）属于 Smacoviridae 聚类，UJSL005（MN604398）与 pCPa-like（pCRESS4-8）聚类密切相关，UJSL006（MN621480）聚类到 pPAPh2-like（pCRESS9）病毒分支。
结论
揭示了来自中国四川成都的森林麝鹿粪便样本中的病毒组，进一步阐明了森林麝鹿肠道内病毒的多样性。
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