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形成生物膜的葡萄球菌牛亚临床乳腺炎分离株的侵袭潜力。

Invasive potential of biofilm-forming Staphylococci bovine subclinical mastitis isolates.

作者信息

Oliveira Manuela, Bexiga Ricardo, Nunes Sandro Filipe, Vilela Cristina Lobo

机构信息

CIISA, Faculty of Veterinary Medicine, Technical University of Lisbon, 1300-477 Lisbon, Portugal.

出版信息

J Vet Sci. 2011 Mar;12(1):95-7. doi: 10.4142/jvs.2011.12.1.95.

Abstract

Staphylococcus (S.) aureus is a common infectious agent of bovine chronic mastitis, a disease that is difficult to eradicate. The abilities of staphylococci to be internalized and form a biofilm can contribute to host immunological defence evasion that subsequently impairs antimicrobial therapy. The invasive capability of six S. aureus field isolates with different biofilmforming profiles was compared in vitro using a bovine mammary epithelial cell line. This was further confirmed in primary cell cultures using fluorescent rRNA probes against S. aureus. The results suggest that S. aureus invasion levels are not related to biofilm formation.

摘要

金黄色葡萄球菌是牛慢性乳腺炎的常见感染源,这种疾病难以根除。葡萄球菌内化并形成生物膜的能力可能有助于逃避宿主免疫防御,进而削弱抗菌治疗效果。使用牛乳腺上皮细胞系在体外比较了六种具有不同生物膜形成特征的金黄色葡萄球菌田间分离株的侵袭能力。使用针对金黄色葡萄球菌的荧光rRNA探针在原代细胞培养中进一步证实了这一点。结果表明,金黄色葡萄球菌的侵袭水平与生物膜形成无关。

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