Kozar Rosemary A, Santora Rachel J, Poindexter Brian J, Milner Stephen M, Bick Roger J
Eplasty. 2011 Feb 23;11:e8.
To determine alterations in quantities and distributions of natural antimicrobials following ischemia-reperfusion injury. We hypothesized that these compounds would be upregulated in areas of small intestine where changes in permeability and cellular disruption were likely and where protective mechanisms would be initiated.
Rats with ischemia-reperfusion underwent superior mesenteric artery clamping and reperfusion. Shams were subjected to laparotomy but no clamping. Ileum and jejunum were harvested and sectioned, and subjected to fluorescence deconvolution microscopy for determinations of content and localization of rat beta defensins, 1, 2, 3; rat neutrophil protein-1; and cathelicidin LL-37. Modeling was performed to determine cellular location of antimicrobials.
Ischemia-reperfusion increased neutrophil defensin alpha (RNP-1) in jejunum; rat beta defensin 1 was increased 2-fold in ileal mucosa and slightly reduced in jejunal mucosa; rat beta defensin 2 was reduced by ischemia-reperfusion in ileum, but slightly increased in jejunum; rat beta defensin 3 was concentrated in the muscularis externa and myenteric plexus of the jejunum; ischemia-reperfusion did not alter cathelicidin LL-37 content in the small intestine, although a greater concentration was seen in jejunum compared with ileum.
Ischemia-reperfusion injury caused changes in antimicrobial content in defined areas, and these different regulations might reflect the specific roles of jejunum versus ileum.
确定缺血再灌注损伤后天然抗菌物质的数量和分布变化。我们假设这些化合物会在小肠中通透性改变和细胞破坏可能发生且保护机制会启动的区域上调。
对大鼠进行肠系膜上动脉夹闭和再灌注以造成缺血再灌注损伤。假手术组仅进行剖腹手术但不夹闭血管。采集回肠和空肠并进行切片，然后通过荧光去卷积显微镜确定大鼠β防御素1、2、3、大鼠中性粒细胞蛋白-1和cathelicidin LL-37的含量及定位。通过建模确定抗菌物质的细胞位置。
缺血再灌注使空肠中的中性粒细胞防御素α（RNP-1）增加；大鼠β防御素1在回肠黏膜中增加了2倍，在空肠黏膜中略有减少；大鼠β防御素2在回肠中因缺血再灌注而减少，但在空肠中略有增加；大鼠β防御素3集中在空肠的外肌层和肌间神经丛；缺血再灌注并未改变小肠中cathelicidin LL-37的含量，尽管空肠中的浓度比回肠中更高。
缺血再灌注损伤导致特定区域抗菌物质含量发生变化，这些不同的调节可能反映了空肠与回肠的特定作用。
