Algae R&D Centre, School of Biological Sciences & Biotechnology, Murdoch University, Murdoch, WA, Australia.
Appl Microbiol Biotechnol. 2011 May;90(4):1399-407. doi: 10.1007/s00253-011-3174-x. Epub 2011 Mar 3.
The effects of changes in CO(2) and pH on biomass productivity and carbon uptake of Pleurochrysis carterae and Emiliania huxleyi in open raceway ponds and a plate photobioreactor were studied. The pH of P. carterae cultures increased during day and decreased at night, whereas the pH of E. huxleyi cultures showed no significant diurnal changes. P. carterae coccolith production occurs during the dark period, whereas in E. huxleyi, coccolith production is mainly during the day. Addition of CO(2) at constant pH (pH-stat) resulted in an increase in P. carterae biomass and coccolith productivity, while CO(2) addition lowered E. huxleyi biomass and coccolith production. Neither of these algae could grow at less than pH 7.5. Species-specific diurnal pH and pCO(2) variations could be indicative of significant differences in carbon uptake between these two species. While E. huxleyi has been suggested to be predominantly a bicarbonate user, our results indicate that P. carterae may be using CO(2) as the main C source for photosynthesis and calcification.
研究了 CO(2) 和 pH 值变化对开放跑道池和板式光生物反应器中拟菱形藻和赫氏海链藻生物量生产力和碳吸收的影响。在白天，拟菱形藻培养液的 pH 值升高，而晚上则降低，而赫氏海链藻培养液的 pH 值没有明显的日变化。拟菱形藻的颗石藻生产发生在黑暗期，而在赫氏海链藻中，颗石藻生产主要发生在白天。在恒定 pH 值（pH -stat）下添加 CO(2) 会导致拟菱形藻生物量和颗石藻生产力增加，而 CO(2) 会降低赫氏海链藻生物量和颗石藻生产。这两种藻类都不能在 pH 值低于 7.5 的条件下生长。这两个物种特定的昼夜 pH 值和 pCO(2) 变化可能表明它们之间的碳吸收存在显著差异。虽然已经有人认为赫氏海链藻主要是碳酸氢盐的使用者，但我们的结果表明，拟菱形藻可能将 CO(2) 用作光合作用和钙化的主要 C 源。
