Raskó I, Burg K, Dallmann L
Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary.
Theor Appl Genet. 1976 Jul;48(4):157-62. doi: 10.1007/BF00527366.
The temporal sequence of mutation to 6-thioguanine resistance was studied in Chinese hamster ovary cells synchronised in S phase. The number of resistant mutants induced following exposure to pulses of bromodeoxyuridine and subsequent selection was significantly greater if BUdR pulses were administered during the first, rather than the second half of S phase. The results are interpreted as indicating that the gene responsible for 6-thioguanine resistance is replicated during the first half of S phase.
在中国仓鼠卵巢细胞中研究了对6-硫鸟嘌呤抗性的突变时间序列，这些细胞在S期被同步化。如果在S期的前半段而非后半段给予溴脱氧尿苷脉冲，那么在暴露于溴脱氧尿苷脉冲并随后进行选择后诱导产生的抗性突变体数量会显著更多。这些结果被解释为表明负责6-硫鸟嘌呤抗性的基因在S期的前半段进行复制。
