Department of Nuclear Medicine, University Hospital Essen, University of Duisburg-Essen, Hufelandstraße 55, 45122 Essen, Germany.
Eur J Nucl Med Mol Imaging. 2011 Jul;38(7):1224-36. doi: 10.1007/s00259-011-1760-x. Epub 2011 Mar 3.
By targeting somatostatin receptors (sst) radiopeptides have been established for both diagnosis and therapy. For physiologically normal human tissues the study provides a normative database of maximum standardized uptake value (SUV(max)) and sst mRNA.
A total of 120 patients were subjected to diagnostic (68)Ga-DOTATOC positron emission tomography (PET)/CT (age range 19-83 years). SUV(max) values were measured in physiologically normal tissues defined by normal morphology, absence of surgical intervention and absence of metastatic spread during clinical follow-up. Expression of sst subtypes (sst1-sst5) was measured independently in pooled adult normal human tissue by real-time reverse transcriptase polymerase chain reaction (RT-PCR).
SUV(max) revealed a region-specific pattern (e.g., mean ± SD, spleen 31.1 ± 10.9, kidney 16.9 ± 5.3, liver 12.8 ± 3.6, stomach 7.0 ± 3.1, head of pancreas 6.2 ± 2.3, small bowel 4.8 ± 1.8, thyroid 4.7 ± 2.2, bone 3.9 ± 1.3, large bowel 2.9 ± 0.8, muscle 2.1 ± 0.5, parotid gland 1.9 ± 0.6, axillary lymph node 0.8 ± 0.3 and lung 0.7 ± 0.3). SUV(max) was age independent. Gender differences were evident within the thyroid (female/male: 3.7 ± 1.6/5.5 ± 2.4, p < 0.001; Mann-Whitney U test) and the pancreatic head (5.5 ± 1.9/6.9 ± 2.2, p < 0.001). The sst mRNA was widely expressed and heterogeneous, showing sst1 to be most abundant. SUV(max) values exclusively correlated with sst2 expression (r = 0.846, p < 0.001; Spearman rank correlation analysis), whereas there was no correlation of SUV(max) with the expression of the other four subtypes.
In normal human tissues (68)Ga-DOTATOC imaging has been related to the expression of sst2 at the level of mRNA. The novel normative database may improve diagnostics, monitoring and therapy of sst-expressing tumours or inflammation on a molecular basis.
通过靶向生长抑素受体（sst），放射性肽已被用于诊断和治疗。对于生理正常的人体组织，该研究提供了最大标准化摄取值（SUV(max)）和 sst mRNA 的正常数据库。
共对 120 名患者进行了（68）Ga-DOTATOC 正电子发射断层扫描（PET）/CT 诊断（年龄范围 19-83 岁）。在形态正常、无手术干预且临床随访期间无转移扩散的生理正常组织中测量 SUV(max)值。通过实时逆转录聚合酶链反应（RT-PCR）在 pooled adult normal human tissue 中独立测量 sst 亚型（sst1-sst5）的表达。
SUV(max)显示出区域特异性模式（例如，平均值±标准差，脾脏 31.1±10.9，肾脏 16.9±5.3，肝脏 12.8±3.6，胃 7.0±3.1，胰头 6.2±2.3，小肠 4.8±1.8，甲状腺 4.7±2.2，骨 3.9±1.3，大肠 2.9±0.8，肌肉 2.1±0.5，腮腺 1.9±0.6，腋窝淋巴结 0.8±0.3，肺 0.7±0.3）。SUV(max)与年龄无关。甲状腺内存在性别差异（女性/男性：3.7±1.6/5.5±2.4，p<0.001；Mann-Whitney U 检验）和胰头（5.5±1.9/6.9±2.2，p<0.001）。sst mRNA 广泛表达且具有异质性，其中 sst1 最为丰富。SUV(max)值仅与 sst2 表达相关（r=0.846，p<0.001；Spearman 秩相关分析），而与其他四种亚型的表达无相关性。
在正常人体组织中，（68）Ga-DOTATOC 成像与 sst2 在 mRNA 水平的表达有关。新的正常数据库可能会提高基于分子的 sst 表达肿瘤或炎症的诊断、监测和治疗。
