Fan H, Gulley M L
Department of Pathology, University of Texas Health Science Center, Sun Antonio, TX.
Methods Mol Med. 2001;49:1-4. doi: 10.1385/1-59259-081-0:1.
In routine histopathology, most tissues are fixed in formalin and embedded in paraffin for long-term preservation. DNA can be extracted from these tissues for subsequent molecular analysis by amplification methods. We describe herein a protocol for DNA preparation from paraffin-embedded tissues based on published procedures (1-3). In brief, tissue sections are placed into microfuge tubes, then deparaffinized with xylene. The xylene is removed with ethanol washes, and the tissue is treated with proteinase K to make DNA available for amplification.
在常规组织病理学中，大多数组织用福尔马林固定并包埋在石蜡中以长期保存。可从这些组织中提取DNA，以便随后通过扩增方法进行分子分析。我们在此描述一种基于已发表程序（1-3）从石蜡包埋组织中制备DNA的方案。简而言之，将组织切片放入微量离心管中，然后用二甲苯脱石蜡。通过乙醇洗涤去除二甲苯，并用蛋白酶K处理组织以使DNA可用于扩增。
