从巴基斯坦拉合尔的两起小型暴发中分离并鉴定出两种新型戊型肝炎病毒基因型 1 株。

Isolation and characterization of two new hepatitis E virus genotype 1 strains from two mini-outbreaks in Lahore, Pakistan.

机构信息

National Centre of Excellence in Molecular Biology, Lahore-53700, University of the Punjab, Lahore, Pakistan.

出版信息

Virol J. 2011 Mar 4;8:94. doi: 10.1186/1743-422X-8-94.

DOI:10.1186/1743-422X-8-94

PMID:21371336

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3056816/

Abstract

BACKGROUND

Pakistan is a highly endemic area for hepatitis E virus (HEV) infection. The aim of the current study was to isolate and characterize strains of HEV in two mini outbreaks.

RESULTS

RNA was extracted and reverse transcribed to cDNA. Nested PCR was done for the detection of HEV RNA. The positive bands were eluted, cloned in TA vector and sequenced in both directions using genetic Analyzer (Applied Biosystem Inc, USA). Phylogenetic analysis was done using MEGA4 software. We isolated two new HEV genotype-1 strains from Lahore, Pakistan, based on cloning and sequencing of ORF2 region.

CONCLUSIONS

Our study suggest that both the human HEV strains were closely related to the Sar-55 but different from the Abb-2B and 87-Pakistan-B HEV isolates sharing 88-91% sequence identity to Pakistani isolate Sar-55. These results indicated that Sar-55 is the main endemic HEV strain in various areas of the country.

摘要

背景

巴基斯坦是戊型肝炎病毒（HEV）感染的高度流行地区。本研究的目的是在两次小型暴发中分离和鉴定 HEV 株。

结果

提取 RNA 并反转录为 cDNA。进行巢式 PCR 检测 HEV RNA。洗脱阳性条带，克隆到 TA 载体中，并使用遗传分析仪（Applied Biosystem Inc，美国）双向测序。使用 MEGA4 软件进行系统发育分析。我们从巴基斯坦拉合尔分离出两种新的 HEV 基因型 1 株，基于 ORF2 区的克隆和测序。