Morimoto Kazuhide, Shimizu Madoka, Kurose Tomoyasu, Nakatani Keiji, Akita Shinji, Shinozuka Yasunori, Isobe Naoki
Hiroshima Prefectural Technology Research Institute, Livestock Technology Research Center, 584 Nanatsuka-cho, Shobara, Hiroshima, 727-0023 Japan.
Hiroshima Prefectural West Livestock Health & Hygiene Service Center, 1-15, Saijogojo-cho, Higashihiroshima, Hiroshima 739-0013 Japan.
J Dairy Res. 2011 May;78(2):149-53. doi: 10.1017/S0022029911000069. Epub 2011 Mar 4.
An enterotoxigenic Escherichia coli (ETEC) vaccine designed to prevent diarrhoea was inoculated into dairy cows, and the occurrence of clinical mastitis was investigated for 2 years. Half of 480 cows in five farms were subcutaneously inoculated with ETEC vaccine (Imocolibov) twice with a 1-month interval in 2007 and 2008. Fisher's exact test and survival (time to event) analysis with the log-rank test were used to compare vaccinates and controls. In 2007, there was no significant difference in the incidence rate of mastitis between vaccinate (20.3%) and control (17.1%) cows. The rate of death or culling due to mastitis was lower in vaccinated cows (7.4%) than in control cows (29.2%, P=0.07, Fisher's exact test; P=0.02, log-rank test). In 2008, there was no significant difference in both the incidence rate of mastitis and the rate of death or culling due to mastitis. Milk productivity was compared between vaccinates and controls in one farm. Multi-way analysis of variance (ANOVA) was performed for the amount of 4% fat-corrected milk, and there was no significant difference between vaccinates and controls. These results suggest that ETEC vaccine inoculation reduces death or culling due to mastitis, whereas no preventive effect on the development of mastitis was observed.
一种旨在预防腹泻的产肠毒素大肠杆菌（ETEC）疫苗被接种到奶牛体内，并对临床乳腺炎的发生情况进行了为期2年的调查。2007年和2008年，五个农场的480头奶牛中有一半皮下接种了ETEC疫苗（Imocolibov），间隔1个月接种两次。采用Fisher精确检验和对数秩检验的生存（事件发生时间）分析来比较接种疫苗组和对照组。2007年，接种疫苗的奶牛（20.3%）和对照奶牛（17.1%）的乳腺炎发病率没有显著差异。接种疫苗的奶牛因乳腺炎导致的死亡或淘汰率（7.4%）低于对照奶牛（29.2%，Fisher精确检验P=0.07；对数秩检验P=0.02）。2008年，乳腺炎发病率以及因乳腺炎导致的死亡或淘汰率均无显著差异。在一个农场中比较了接种疫苗组和对照组的产奶量。对4%脂肪校正乳的产量进行了多因素方差分析（ANOVA），接种疫苗组和对照组之间没有显著差异。这些结果表明，接种ETEC疫苗可降低因乳腺炎导致的死亡或淘汰率，而未观察到对乳腺炎发生的预防作用。
