Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA.
Anal Biochem. 2011 Jul 1;414(1):99-102. doi: 10.1016/j.ab.2011.02.039. Epub 2011 Mar 1.
We have developed a fibrinogen-specific sandwich enzyme-linked immunosorbent assay (ELISA) microarray assay for use in qualitatively distinguishing between blood plasma and serum samples. Three capture antibodies (49D2, HPA001900, and F8512) were evaluated in conjunction with 1D6 as the detection antibody. The data show that 49D2 and (to a lesser extent) F8512 successfully identify previously unknown plasma and serum samples based on approximately a 28-fold difference in signal intensity between the sample types. This assay has utility in rapidly identifying previously archived clinical samples with incomplete annotation in a high-throughput manner prior to proteomic analyses.
我们开发了一种纤维蛋白原特异性夹心酶联免疫吸附试验（ELISA）微阵列分析方法，用于定性区分血浆和血清样本。三种捕获抗体（49D2、HPA001900 和 F8512）与 1D6 作为检测抗体一起进行了评估。数据表明，49D2 和（在较小程度上）F8512 成功地根据样品类型之间约 28 倍的信号强度差异，识别出以前未知的血浆和血清样本。该检测方法可在进行蛋白质组学分析之前，快速识别以前存档的临床样本，并以高通量的方式对其进行鉴定。
