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用于改进基于纸的酶联免疫吸附测定中蛋白质固定的原子转移自由基聚合物改性纸。

Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA.

作者信息

Qi Lu, Zhang Aihong, Wang Yu, Liu Long, Wang Xinghe

机构信息

1Phase I Clinical Trial Center, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038 China.

Institute of Chemical Defense, Beijing, 102205 China.

出版信息

BMC Chem. 2019 Aug 22;13(1):110. doi: 10.1186/s13065-019-0622-7. eCollection 2019 Dec.

DOI:10.1186/s13065-019-0622-7
PMID:31463479
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6706939/
Abstract

A newly modified paper-based enzyme-linked immunosorbent assay (P-ELISA) was established by immobilizing more proteins on the paper surface through an atom transfer radical polymerization (ATRP) reaction. In addition, introducing graphene oxide (GO) sheets, Au nanoparticles (AuNps) and two primary antibodies (Ab1s) led to signal amplification and cost reduction.

摘要

通过原子转移自由基聚合（ATRP）反应将更多蛋白质固定在纸表面，建立了一种新改良的基于纸的酶联免疫吸附测定法（P-ELISA）。此外，引入氧化石墨烯（GO）片、金纳米颗粒（AuNps）和两种一抗（Ab1s）可实现信号放大并降低成本。

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