从松材线虫中克隆和鉴定钙网蛋白 cDNA。
Molecular cloning and characterization of a calreticulin cDNA from the pinewood nematode Bursaphelenchus xylophilus.
机构信息
Laboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642, China.
出版信息
Exp Parasitol. 2011 Jun;128(2):121-6. doi: 10.1016/j.exppara.2011.02.017. Epub 2011 Mar 1.
The cloning and characterization of a cDNA encoding a calreticulin from the pinewood nematode Bursaphelenchus xylophilus is described herein. The full-length cDNA (Bx-crt-1) contained a 1200 bp open reading frame that could be translated to a 399 amino acid polypeptide. The deduced protein contained highly conserved regions of a calreticulin gene and had 66.2-70.1% amino acid sequence identity to other calreticulin sequences from nematodes. RNAi, RT-PCR amplification, and southern blot suggest that Bx-crt-1 may be important for the development of B. xylophilus.
本文描述了从松材线虫（Bursaphelenchus xylophilus）中克隆和鉴定钙网蛋白 cDNA 的过程。全长 cDNA（Bx-crt-1）包含一个 1200bp 的开放阅读框，可翻译成一个 399 个氨基酸的多肽。推导的蛋白含有钙网蛋白基因的高度保守区域，与其他线虫的钙网蛋白序列具有 66.2-70.1%的氨基酸序列同一性。RNAi、RT-PCR 扩增和 Southern blot 表明，Bx-crt-1 可能对松材线虫的发育很重要。
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