Centre for Functional Ecology, Associate Laboratory TERRA, Department of Life Sciences, University of Coimbra, Calçada Martins de Freitas, Coimbra, 3000-456, Portugal.
CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Rua Larga, Polo I, Coimbra, 3004-504, Portugal.
BMC Plant Biol. 2024 Feb 9;24(1):98. doi: 10.1186/s12870-024-04771-9.
Pine wilt disease (PWD) is a devastating forest disease caused by the pinewood nematode (PWN), Bursaphelenchus xylophilus, a migratory endoparasite that infects several coniferous species. During the last 20 years, advances have been made for understanding the molecular bases of PWN-host trees interactions. Major advances emerged from transcriptomic and genomic studies, which revealed some unique features related to PWN pathogenicity and constituted fundamental data that allowed the development of postgenomic studies. Here we review the proteomic approaches that were applied to study PWD and integrated the current knowledge on the molecular basis of the PWN pathogenicity. Proteomics has been useful for understanding cellular activities and protein functions involved in PWN-host trees interactions, shedding light into the mechanisms associated with PWN pathogenicity and being promising tools to better clarify host trees PWN resistance/susceptibility.
松材线虫萎蔫病（PWD）是一种由松材线虫（PWN）引起的毁灭性森林病害，是一种迁移性内寄生虫，感染多种针叶树种。在过去的 20 年中，人们对松材线虫与宿主树木相互作用的分子基础有了更多的了解。转录组学和基因组学研究取得了重大进展，揭示了一些与松材线虫致病性相关的独特特征，为后生学研究的发展提供了基础数据。在这里，我们回顾了应用于研究 PWD 的蛋白质组学方法，并整合了当前关于松材线虫致病性的分子基础的知识。蛋白质组学有助于了解与松材线虫-宿主树木相互作用相关的细胞活动和蛋白质功能，为与松材线虫致病性相关的机制提供了启示，并有望成为更好地阐明宿主树木对松材线虫抗性/易感性的工具。
