Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu Province 226001, PR China.
FEBS Lett. 2011 Apr 6;585(7):1014-20. doi: 10.1016/j.febslet.2011.02.034. Epub 2011 Mar 1.
Glial fibrillary acidic protein (GFAP) is a principal intermediate filament in mature astrocytes of the central nervous system (CNS), and the regulation of GFAP transcription has not been well understood yet. In the present study, we reported paired box 3 protein (Pax3) as a novel regulator of GFAP transcription, which could bind the promoter region of GFAP and down regulate the GFAP level during the serum-induced astrocyte differentiation of neural stem cells (NSCs). Moreover, the overexpression and suppression of Pax3 could inhibit and promote NSC differentiation, respectively. These data suggest that Pax3 negatively regulates GFAP expression during astrocyte differentiation in vitro.
胶质纤维酸性蛋白（GFAP）是中枢神经系统（CNS）成熟星形胶质细胞中的主要中间丝，但其转录调控尚不清楚。本研究报道配对盒蛋白 3（Pax3）是 GFAP 转录的新调节因子，它可以与 GFAP 启动子区域结合，并在神经干细胞（NSC）的血清诱导星形胶质细胞分化过程中下调 GFAP 水平。此外，Pax3 的过表达和抑制分别可以抑制和促进 NSC 的分化。这些数据表明，Pax3 在体外星形胶质细胞分化过程中负调控 GFAP 的表达。
