The University of Queensland, Delivery of Drugs and Genes Group (D(2)G(2)), Australian Institute for Bioengineering and Nanotechnology, Brisbane.
J Control Release. 2011 Jun 30;152(3):349-55. doi: 10.1016/j.jconrel.2011.02.026. Epub 2011 Mar 1.
Dry-coated microprojections can deliver vaccine to abundant antigen-presenting cells in the skin and induce efficient immune responses and the dry-coated vaccines are expected to be thermostable at elevated temperatures. In this paper, we show that we have dramatically improved our previously reported gas-jet drying coating method and greatly increased the delivery efficiency of coating from patch to skin to from 6.5% to 32.5%, by both varying the coating parameters and removing the patch edge. Combined with our previous dose sparing report of influenza vaccine delivery in a mouse model, the results show that we now achieve equivalent protective immune responses as intramuscular injection (with the needle and syringe), but with only 1/30th of the actual dose. We also show that influenza vaccine coated microprojection patches are stable for at least 6 months at 23°C, inducing comparable immunogenicity with freshly coated patches. The dry-coated microprojection patches thus have key and unique attributes in ultimately meeting the medical need in certain low-resource regions with low vaccine affordability and difficulty in maintaining "cold-chain" for vaccine storage and transport.
干涂层微针可将疫苗递送至皮肤中丰富的抗原呈递细胞，诱导有效的免疫反应，且干涂层疫苗有望在高温下保持热稳定性。在本文中，我们展示了对先前报道的气体喷射干燥涂层方法进行了显著改进，通过改变涂层参数和去除贴片边缘，将涂层从贴片到皮肤的递送效率从 6.5%大幅提高到 32.5%。结合我们之前在小鼠模型中进行流感疫苗给药的剂量节省报告，结果表明，我们现在实现了与肌肉注射（使用针和注射器）相当的保护性免疫反应，但实际剂量仅为 1/30。我们还表明，流感疫苗涂层微针贴片在 23°C 下至少稳定 6 个月，与新涂层的贴片具有相当的免疫原性。因此，干涂层微针贴片具有关键且独特的属性，有望满足某些资源匮乏地区的医疗需求，这些地区疫苗可负担性低，且在疫苗储存和运输过程中难以维持“冷链”。
