Department of Pharmaceutics, Utrecht University of Pharmaceutical Sciences, Utrecht University, The Netherlands.
Int J Pharm. 2011 Sep 20;416(2):426-32. doi: 10.1016/j.ijpharm.2011.02.055. Epub 2011 Mar 1.
Dendritic cells (DCs) are key antigen presenting cells that have the unique ability to present antigens on MHC molecules, which can lead to either priming or suppression of T cell mediated immune responses. C-type lectin receptors expressed by DCs are involved in antigen uptake and presentation through recognition of carbohydrate structures on antigens. Here we have explored the feasibility of modification of liposomes with glycans for targeting purposes to boost immune responses. The potential of targeting glycoliposomal constructs to the C-type lectin DC-SIGN on DCs was studied using either PEGylated or non-PEGylated liposomes. Our data demonstrate that formulation of the glycoliposomes as PEGylated negatively affected their potential to target to DCs.
树突状细胞 (DCs) 是关键的抗原呈递细胞，具有在 MHC 分子上呈递抗原的独特能力，这可能导致 T 细胞介导的免疫反应的启动或抑制。DC 表达的 C 型凝集素受体参与通过识别抗原上的碳水化合物结构进行抗原摄取和呈递。在这里，我们探索了用糖来修饰脂质体以用于靶向目的来增强免疫反应的可行性。使用 PEG 化或非 PEG 化的脂质体研究了将糖脂体构建物靶向 DC 上的 C 型凝集素 DC-SIGN 的可能性。我们的数据表明，将糖脂体制剂化为 PEG 化会负面影响它们靶向 DC 的潜力。
