INSERM U710, Montpellier F-34095, France.
Neurobiol Aging. 2012 Feb;33(2):431.e15-25. doi: 10.1016/j.neurobiolaging.2011.01.002. Epub 2011 Mar 2.
We analyzed the cellular distribution of the pancreatic inflammatory protein lithostathine and its receptor EXTL3 in the brain of the lemurian primate Microcebus murinus which develops amyloid deposits along with aging. In adult animals (2-4.5 years old), lithostathine and EXTL3 immunoreactivities were largely distributed in the whole brain, and more intensively in almost all cortical layers and hippocampal formation. Lithostathine was observed in the perikarya and neurites of cortical neurons but also in glial cells in the border of the ventricle and the corpus callosum. In healthy aged animals (8-13 years old), highest densities of lithostathine-containing cells were observed, mainly in occipital and parietal cortex. In aged animals with Aβ deposits, the increase in lithostathine immunoreactivity was lower as compared with aged animals. Noteworthy, lithostathine-immunopositive cells did almost never colocalize with Aβ plaques. In conclusion, lithostathine immunoreactivity in adult Microcebus murinus appeared ubiquitous and particularly in visual, sensorial, and cognitive brain areas. Immunoreactivity increased with aging and appeared markedly affected in neuropathological conditions. Its possible neuroprotection or neurodegeneration role in Alzheimer pathology deserves therefore to be investigated.
我们分析了胰腺炎症蛋白 lithostathine 及其受体 EXTL3 在灵长类动物 lemurian 原代动物 Microcebus murinus 大脑中的细胞分布情况，该动物随着年龄的增长会产生淀粉样沉积物。在成年动物（2-4.5 岁）中，lithostathine 和 EXTL3 免疫反应性广泛分布于整个大脑，在几乎所有皮质层和海马结构中更为强烈。lithostathine 存在于皮质神经元的胞体和突起中，但也存在于脑室和胼胝体边界的神经胶质细胞中。在健康的老年动物（8-13 岁）中，观察到含有 lithostathine 的细胞密度最高，主要在枕叶和顶叶皮层。在有 Aβ 沉积的老年动物中，与老年动物相比，lithostathine 免疫反应性的增加较低。值得注意的是，lithostathine-免疫阳性细胞几乎从不与 Aβ 斑块共定位。总之，成年 Microcebus murinus 的 lithostathine 免疫反应性似乎无处不在，特别是在视觉、感觉和认知大脑区域。免疫反应性随年龄增长而增加，在神经病理学条件下明显受到影响。因此，值得研究其在阿尔茨海默病病理中的神经保护或神经退行性作用。
