Guerreiro da Silva André Vilella, Nakamura Mary Uchiyama, Cordeiro José Antônio, Guerreiro da Silva João Bosco, Mendes Gloria E F, Burdmann Emmanuel A
Universidade Federal de São Paulo, Brazil.
Forsch Komplementmed. 2011;18(1):10-4. doi: 10.1159/000323566. Epub 2011 Jan 31.
This study sought to determine if acupuncture in LI4 and SP6, or in sacral points could harm the pregnancy outcome in Wistar rats as is believed according to traditional knowledge.
48 pregnant Wistar rats were randomly divided into 4 groups: total control where the rats were left in cages without manipulation; anesthetized control where the rats were manipulated and anesthetized but did not received electroacupuncture; peripheral points and sacral points were the rats were anesthetized and received 4 acupuncture points - LI4-SP6 and BL27-28, respectively. The primary end point was embryonic loss after implantation, defined as (number of implantations - number of embryos in development) × 100 / number of implantations. Other evaluated parameters were fetal death, abortions, number of fetuses, and resorptions, resorption index (number of resorptions / total of implantations), maternal weight gain, and fetal weight.
There were no differences in embryonic loss after implantation (p = 0.45), fetal death (p = 1), abortions (p = 1), number of fetuses (p = 0.79), resorptions (p = 0.3), and resorption index (p = 0.45). There were differences in maternal weight gain and fetal weight, but they seemed unrelated to the treatment.
We found no evidence that acupuncture in LI4-SP6 and sacral points could be harmful to the pregnancy outcome in Wistar rats.
本研究旨在确定如传统认知所认为的那样，针刺手阳明大肠经的合谷穴（LI4）和足太阴脾经的三阴交穴（SP6）或骶部穴位是否会损害Wistar大鼠的妊娠结局。
将48只妊娠Wistar大鼠随机分为4组：完全对照组，将大鼠置于笼中不进行任何操作；麻醉对照组，对大鼠进行操作并麻醉，但不接受电针治疗；外周穴位组和骶部穴位组，分别对大鼠进行麻醉，并针刺4个穴位——LI4 - SP6和BL27 - 28。主要终点是着床后的胚胎丢失，定义为（着床数 - 发育中的胚胎数）×100 / 着床数。其他评估参数包括胎儿死亡、流产、胎儿数量、吸收情况、吸收指数（吸收数 / 总着床数）、母体体重增加和胎儿体重。
着床后的胚胎丢失（p = 0.45）、胎儿死亡（p = 1）、流产（p = 1）、胎儿数量（p = 0.79）、吸收情况（p = 0.3）和吸收指数（p = 0.45）均无差异。母体体重增加和胎儿体重存在差异，但似乎与治疗无关。
我们没有发现证据表明针刺LI4 - SP6和骶部穴位会对Wistar大鼠的妊娠结局有害。
