贝伐单抗（阿瓦斯汀®）对离体大鼠视网膜电图的影响。
Effects of bevacizumab (Avastin®) on the electroretinogram of isolated rat retina.
机构信息
Laboratoire de Biophysique des Handicaps Sensoriels EA 2667, UFR Pharmacie, Clermont Université, Clermont-Ferrand, France.
出版信息
Ophthalmic Res. 2011;46(3):145-51. doi: 10.1159/000324136. Epub 2011 Mar 4.
AIM
To evaluate the in vitro effects of bevacizumab (Avastin®) on the electroretinogram (ERG) in rats using a model of isolated perfused retina ERG recording.
METHODS
Retinas were isolated from rat eyes and placed in a chamber continuously perfused with a nutrient solution. The ERG was recorded every 3 min. Once the ERG b-wave amplitude was at a steady state, bevacizumab was added at concentrations of 0.25 and 0.5 mg/ml to the perfusion medium for 3 h.
RESULTS
We observed no effect on ERG amplitudes or kinetics when bevacizumab was added to the perfusion medium. In addition, we found no significant differences in the survival curves of the b-wave and PIII wave during the application of bevacizumab between bevacizumab-exposed retinas and control retinas.
CONCLUSIONS
We demonstrate that bevacizumab has no in vitro toxic effects on the ERG of isolated perfused rat retina. Our study supports the retinal safety of bevacizumab with respect to retinal function.
目的
采用离体灌流视网膜电图（ERG）记录模型，评估贝伐单抗（Avastin®）对大鼠 ERG 的体外作用。
方法
从大鼠眼分离出视网膜，置于持续用营养溶液灌流的腔室内。每 3 分钟记录一次 ERG。一旦 ERG b 波振幅达到稳定状态，将贝伐单抗以 0.25 和 0.5 mg/ml 的浓度加入灌流介质中，持续 3 小时。
结果
当贝伐单抗加入灌流介质中时，对 ERG 振幅或动力学没有影响。此外，在贝伐单抗作用期间，暴露于贝伐单抗的视网膜和对照视网膜之间 b 波和 PIII 波的生存曲线没有显著差异。
结论
我们证明贝伐单抗对离体灌流大鼠视网膜的 ERG 没有体外毒性作用。我们的研究支持贝伐单抗在视网膜功能方面的视网膜安全性。
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