比较两种不同灌流液对离体灌流脊椎动物视网膜的影响。

Comparing the effects of two different irrigation solutions on an isolated perfused vertebrate retina.

机构信息

Department of Ophthalmology I, Eberhard Karls University of Tübingen, Tübingen, Germany.

出版信息

Ophthalmic Res. 2012;48(2):59-66. doi: 10.1159/000335365.

DOI:10.1159/000335365

PMID:22472946

Abstract

PURPOSE

To compare the effect of a taurine-containing intraocular irrigation solution (PuriProtect TM) to a standard irrigation solution (BSS TM) we evaluated the retinal function using an electroretinogram (ERG) and analyzed the survival of retinal ganglion cells on isolated whole mount retinas.

MATERIALS AND METHODS

During ERG recordings, each irrigation solution was superfused for 45 min with the relevant irrigation solution. To investigate the effects on photoreceptor function, 1 m M asparate was added to obtain a-waves.The recovery of the a- and b-wave was monitored after superfusing the retinas with standard medium again. To evaluate the percentage of dead ganglion cells, retinas were stored for 24 h at 4°C in darkness and after staining the retinas with ethidium homodimer-1 the retinas were analyzed using fluorescence microscopy.

RESULTS

The application of standard medium supplemented with 2 m M taurine resulted in a significant increase of the b-wave amplitude compared to standard medium alone. The a-wave amplitudes showed no significant changes under taurine supplementation. Compared to standard medium BSS showed no significant decrease in b-wave amplitudes, but a significant decrease ina-wave amplitudes. In contrast to BSS there were no significant changes in the a- or b-wave amplitudes detectable after the application of PuriProtect. At the end of the washout period no significant changes in a- or b-wave amplitudes were recorded for any tested irrigation solution. Retinas stored for 24 h in PuriProtect or in standard medium with taurine had a statistically significant smaller amount of dead cells than retinas stored in standard medium without taurine supplementation.

CONCLUSIONS

BSS does not seem to be an ideal irrigation solution, because it compromises the a-wave in the ERG. In contrast to BSS, PuriProtect showed no significant impact on the ERG and showed a better long-term effect on ganglion cell survival. Taurine supplementation,therefore, seems to be neuroprotective and its supplementation to an intraocular irrigation solution favorable for the retina.

摘要

目的

为了比较一种含牛磺酸的眼内灌洗液（PuriProtectTM）和一种标准灌洗液（BSSTM）的效果，我们使用视网膜电图（ERG）评估视网膜功能，并在分离的全视网膜上分析神经节细胞的存活情况。

材料和方法

在 ERG 记录过程中，每种灌洗液都用相关灌洗液进行 45 分钟的超灌洗。为了研究对光感受器功能的影响，加入 1m M 天冬氨酸以获得 a 波。在用标准培养基再次超灌洗视网膜后，监测 a 波和 b 波的恢复情况。为了评估死亡的神经节细胞的百分比，视网膜在 4°C黑暗中储存 24 小时，然后用 ethidium homodimer-1 染色视网膜，使用荧光显微镜分析视网膜。

结果

与单独使用标准培养基相比，标准培养基中添加 2m M 牛磺酸可显著增加 b 波幅度。牛磺酸补充后，a 波幅度没有显著变化。与 BSS 相比，BSS 中 b 波幅度没有显著降低，但 a 波幅度显著降低。与 BSS 相比，应用 PuriProtect 后，a 波或 b 波幅度没有明显变化。在冲洗期结束时，任何测试的灌洗液的 a 波或 b 波幅度都没有记录到明显变化。在 PuriProtect 或含牛磺酸的标准培养基中储存 24 小时的视网膜比在不含牛磺酸补充的标准培养基中储存的视网膜的死亡细胞数量明显减少。