Namera Akira, Makita Ryosuke, Saruwatari Tatsuro, Hatano Aiko, Shiraishi Hiroaki, Nagao Masataka
Department of Forensic Medicine, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
Am J Forensic Med Pathol. 2012 Dec;33(4):293-6. doi: 10.1097/PAF.0b013e31820f1514.
A 52-year-old woman was found dead on the floor of the living room on the first floor of a house, which belonged to the man with whom she shared the house. On visiting the site, her clothes were found to be undisturbed. Packages of flunitrazepam (Silece, 2 mg/tablet) and triazolam (Halcion, 0.25 mg/tablet) were found strewn around the victim. Toxicological analysis was performed, and the concentrations of flunitrazepam, triazolam, and their metabolites in the victim's blood and urine were measured by high-performance liquid chromatography coupled with photodiode array and mass spectrometry. A high blood concentration of 7-aminoflunitrazepam was detected (1,270 ng/g), and further metabolites such as 7-acetamidoflunitrazepam, 7-acetamidodesmethylflunitrazepam, and 7-aminodesmethylflunitrazepam were detected in the blood and urine samples. In addition, 4-hydroxytriazolam and α-hydroxytriazolam were detected in her urine at a concentration of 950 and 12,100 ng/mL, respectively.On the basis of the autopsy findings and toxicology results of high concentrations of both flunitrazepam and triazolam derivatives, the cause of death was determined to be acute intoxication from flunitrazepam and triazolam.
一名52岁女性被发现死在一所房子一楼客厅的地板上，这所房子属于与她合住的一名男子。到达现场时，发现她的衣服没有被动过。在受害者周围发现了散落的氟硝西泮（思力斯，2毫克/片）和三唑仑（海乐神，0.25毫克/片）包装。进行了毒理学分析，通过高效液相色谱结合光电二极管阵列和质谱法测量了受害者血液和尿液中氟硝西泮、三唑仑及其代谢物的浓度。检测到血液中7-氨基氟硝西泮浓度很高（1270纳克/克），并且在血液和尿液样本中还检测到了其他代谢物，如7-乙酰氨基氟硝西泮、7-乙酰氨基去甲基氟硝西泮和7-氨基去甲基氟硝西泮。此外，在她的尿液中检测到4-羟基三唑仑和α-羟基三唑仑，浓度分别为950和12100纳克/毫升。根据尸检结果以及氟硝西泮和三唑仑衍生物高浓度的毒理学结果，确定死亡原因是氟硝西泮和三唑仑急性中毒。
